biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 60:329-342, 2016 | DOI: 10.1007/s10535-016-0596-4

Role of foliar application of 24-epibrassinolide in response of peanut seedlings to iron deficiency

Y. L. Song1, Y. J. Dong1,*, X. Y. Tian1, J. Kong1, X. Y. Bai1, L. L. Xu1, Z. L. He2
1 College of Resources and Environment, Shandong Agricultural University, Tai'An, P.R. China
2 Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, USA

Limited information is available on the role of brassinosteroids (BRs) in response of plants to nutrient deficiency. To understand the functions of BRs in response to iron deficiency, we investigated the effect of 24-epibrassinolide (EBR) on activities of ferric-chelate reductase (FCR), H+-ATPase, Ca2+-ATPase, nitrate reductase (NR), antioxidant enzymes, Fe and other minerals content and distribution, chlorophylls, soluble protein, free proline, reactive oxygen species, and malondialdehyde in peanut (Arachis hypogea L.) plants subjected to Fe deficiency (10-5 M Fe(III)-EDTA) with foliar application of EBR (0, 10-8, 5.0×10-8, 10-7, 5.0×10-7, and10-6 M). Results show that EBR increased Fe translocation from roots to shoots and increased Fe content in cell organelles. Activities of antioxidant enzymes increased and so the ability of resistance to oxidative stress was enhanced. As result of enhancement of H+-ATPase and Ca2+-ATPase activities, the inhibition of Fe, Ca, Mg, and Zn uptake and distribution was ameliorated. Chlorophyll, soluble protein, and free proline content also increased and consequently, chlorosis induced by Fe deficiency was alleviated. The results demonstrate that EBR had a positive role in regulating peanut growth and development under Fe deficiency and an optimal concentration appeared to be 10-7 M.

Keywords: antioxidant enzymes; brassinosteroids; chlorophylls; mineral elements; reactive oxygen species; subcellular distribution of Fe
Subjects: brassinosteroids; iron deficiency; iron distribution; chlorophyll; reactive oxygen species; antioxidants; carotenoids; calcium; magnesium; copper; zinc; peanut

Received: December 29, 2014; Revised: July 31, 2015; Accepted: September 22, 2015; Published: June 1, 2016  Show citation

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Song, Y.L., Dong, Y.J., Tian, X.Y., Kong, J., Bai, X.Y., Xu, L.L., & He, Z.L. (2016). Role of foliar application of 24-epibrassinolide in response of peanut seedlings to iron deficiency. Biologia plantarum, 60(2), 329-342. doi: 10.1007/s10535-016-0596-4
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