biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:507-514, 2014 | DOI: 10.1007/s10535-014-0438-1

Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources

J. C. Alvarez-Pizarro1, E. Gomes-Filho1,*, J. T. Prisco1, M. F. Grossi-De-Sá2, O. B. De Oliveira-Neto2,3, R. Da Rocha Fragoso2
1 Departamento de Bioquímica e Biologia Molecular, Instituto Nacional de Ciência e Tecnologia em Salinidade, Universidade Federal do Ceará, Fortaleza, Ceará-Brazil
2 Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Brasília, DF, Brazil
3 Centro Universitário Unieuro, Brasília, DF, Brazil

We studied the influence of inorganic nitrogen sources (NO3 - or NH4 +) and potassium deficiency on expression and activity of plasma membrane (PM) H+-ATPase in sorghum roots. After 15 d of cultivation at 0.2 mM K+, the plants were transferred to solutions lacking K+ for 2 d. Then, K+ depletion assays were performed in the presence or absence of vanadate. Further, PMs from K+-starved roots were extracted and used for the kinetic characterization of ATP hydrolytic activity and the immunodetection of PM H+-ATPase. Two major genes coding PM H+-ATPase (SBA1 and SBA2) were analyzed by real-time PCR. PM H+-ATPase exhibited a higher Vmax and Km in NH4 +-fed roots compared with NO3 - -fed roots. The optimum pH of the enzyme was slightly lower in NO3 - -fed roots than in NH4 +-fed roots. The vanadate sensitivity was similar. The expressions of SBA1 and SBA2 increased in roots grown under NH4 +. Concomitantly, an increased content of the enzyme in PM was observed. The initial rate of K+ uptake did not differ between plants grown with NO3 - or NH4 +, but it was significantly reduced by vanadate in NH4 +-grown plants.

Keywords: ammonium; immunodetection; K+ uptake; nitrate; Sorghum bicolor; vanadate
Subjects: plasma membrane; ATPase; potassium; nitrogen; ammonium; nitrate; vanadate; phylogenetic analysis; sorghum

Received: October 17, 2013; Revised: November 29, 2013; Accepted: January 10, 2014; Published: September 1, 2014  Show citation

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Alvarez-Pizarro, J.C., Gomes-Filho, E., Prisco, J.T., Grossi-De-Sá, M.F., De Oliveira-Neto, O.B., & Da Rocha Fragoso, R. (2014). Plasma membrane H+-ATPase in sorghum roots as affected by potassium deficiency and nitrogen sources. Biologia plantarum, 58(3), 507-514. doi: 10.1007/s10535-014-0438-1
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