biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 58:114-120, 2014 | DOI: 10.1007/s10535-013-0370-9

Growth characteristics and phenylalanine ammonia-lyase activity in peach grafted on different Prunus spp.

I. Dos Santos Pereira1, R. Da Silva Messias2, Â. Diniz Campos3, P. Errea4, L. E. Corrêa Antunes1, J. C. Fachinello5, A. Pina4,*
1 Departamento de Fruticultura, Embrapa Clima Temperado, Pelotas, Brazil
2 Departamento de Biologia Molecular, Embrapa Clima Temperado, Pelotas, Brazil
3 Departamento de Fisiologia Vegetal, Embrapa Clima Temperado, Pelotas, Brazil
4 Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragoza, Spain
5 Departamento de Fitotecnia, Universidade Federal de Pelotas, Pelotas, Brazil

Phenylalanine ammonia lyase (PAL) is a key enzyme in the phenylpropanoid pathway responsible for biosynthesis of many secondary metabolites, such as anthocyanins, flavanols, and lignins. The objective of this work was to determine the effect of different rootstock/scion combinations on Prunus tree growth, PAL gene expression, and PAL activity in order to identify compatibility of different graft combinations. The study was performed with peach (P. persica cv. Chimarrita) grafted on two peach rootstocks (Capdeboscq and Tsukuba1) and one Japanese apricot (P. mume cv. Umezeiro). Two or three years after grafting, the growth of peach scion on Umezeiro was weak and finally the death of some trees occurred. The peach rootstocks induced vigorous growth without any tree loss through the three years of evaluation. However, PAL activity and expression of PAL encoding genes were higher in Umezeiro as compared to the other rootstocks. These results show that the differential gene expression together with the PAL activity is a promising strategy to predict graft incompatibility.

Keywords: phenylpropanoid pathway; Prunus mume; Prunus persica; rootstock-scion compatibility; secondary metabolism
Subjects: phenylalanine ammonia-lyase; grafts; rootstocks; phenylpropanoid pathway; graft compatibility; growth; gene expression; peach

Received: December 21, 2012; Revised: May 20, 2013; Accepted: June 19, 2013; Published: March 1, 2014  Show citation

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Dos Santos Pereira, I., Da Silva Messias, R., Diniz Campos, Â., Errea, P., Corrêa Antunes, L.E., Fachinello, J.C., & Pina, A. (2014). Growth characteristics and phenylalanine ammonia-lyase activity in peach grafted on different Prunus spp. Biologia plantarum, 58(1), 114-120. doi: 10.1007/s10535-013-0370-9
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