biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:99-104, 2014 | DOI: 10.1007/s10535-013-0360-y

Effect of water stress on yield and nutrition quality of tomato plant overexpressing StAPX

W.-H. Sun1,*, X.-Y. Liu2, Y. Wang1, Q. Hua1, X.-M. Song1, Z. Gu1, D.-Z. Pu1
1 College of Food and Biological Engineering, Jiangsu University, Zhenjiang, P.R. China
2 College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, P.R. China

We investigated the effect of water stress on yield and quality of tomato plants overexpressing Solanum lycopersicum thylakoid-bound ascorbate peroxidase gene (StAPX). APX activity, hydrogen peroxide content, net photosynthetic rate of tomato leaves, and yield and nutrition quality of tomato fruits were measured under soil moisture 70, 60, and 50 % of full field capacity. Results show that the capability of APX for scavenging hydrogen peroxide induced by water stress was higher in the transgenic than the wild type (WT) plants. The yield of fruits of the transgenic tomato plants was higher than that of WT plants under water stress and the fruit nutrition quality was not different. These results indicate that overexpression of StAPX might improve water stress tolerance in the transgenic tomato plants.

Keywords: chlorophyll; H2O2 scavenging; net photosynthetic rate; proline; Solanum lycopersicum; sugars; thylakoid-bound ascorbate peroxidase
Subjects: water stress; gene expression; chlorophyll; net photosynthetic rate; hydrogen peroxide; proline; ascorbate peroxidase; ascorbic acid; sugars; proteins; tomato; lycopene

Received: August 23, 2012; Revised: April 14, 2013; Accepted: April 17, 2013; Published: March 1, 2014  Show citation

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Sun, W.-H., Liu, X.-Y., Wang, Y., Hua, Q., Song, X.-M., Gu, Z., & Pu, D.-Z. (2014). Effect of water stress on yield and nutrition quality of tomato plant overexpressing StAPX. Biologia plantarum, 58(1), 99-104. doi: 10.1007/s10535-013-0360-y
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    References

    1. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    2. Chen, G.X., Asada, K.: Ascorbate peroxidases in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. - Plant Cell Physiol. 30: 987-998, 1989.
    3. Chen, X.X., Ma, F.Y., Chu, G.X., Xue, L., Li, Y., Wang, J.J.: [Recent advances on processing tomato water relations]. - Chin. agr. Sci. Bull. 22: 206-208, 2006. [In Chin.]
    4. Danna, C.H., Bartoli, C.G., Sacco, F., Ingala, L.R., Santa-Maria, G.E., Guiamet, J.J., Ugalde, R.A.: Thylakoid-bound ascorbate peroxidase mutant exhibits impaired electron transport and photosynthetic activity. - Plant Physiol. 132: 2116-2125, 2003. Go to original source...
    5. Ferguson, I.B., Watkins, C.B., Harman, J.E.: Inhibition by calcium of senescence of detached cucumber cotyledons. - Plant Physiol. 71: 182-186, 1983. Go to original source...
    6. Hemavathi, Upadhyaya, C.P., Akula, N., Young, K.E., Chun, S.C., Kim, D.H., Park, S.W.: Enhanced ascorbic acid accumulation in transgenic potato confers tolerance to various abiotic stresses. - Biotechnol. Lett. 32: 321-330, 2010. Go to original source...
    7. Horsch, R.B., Fry, J.E., Hoffmann, N.L., Eichholtz, D., Rogers, S.G., Fraley, R.T.: A simple and general method for transferring genes into plants. - Science 227: 1229-1231, 1985. Go to original source...
    8. Jacob, E.J.: Water: under pressure. - Nature 452: 269, 2008. Go to original source...
    9. Lee, Y.P., Kim, S.H., Bang, J.W., Lee, H.S., Kwak, S.S., Kwon, S.Y.: Enhanced tolerance to oxidative stress in transgenic tobacco plants expressing three antioxidant enzymes in chloroplasts. - Plant Cell Rep. 26: 591-598, 2007. Go to original source...
    10. Li, G.L., Wu, H.X., Wen, L., Shao, K., Li, Z.Y., Zhang, S.Y.: [Progress in physiological and molecular mechanism of drought resistant in crops.] - Chin. agr. Sci. Bull. 26: 185-191, 2010. [In Chin.]
    11. Li, Y.H., Liu, Y.J., Xu, X.L., Jin, M., An, L.Z., Zhang, H.: Effect of 2,4-epibrassinolide on drought stress-induced changes in Chorispora bungeana. - Biol. Plant. 56: 192-196, 2012. Go to original source...
    12. Meloni, D.A., Gulotta, M.R., Martínez, C.A., Oliva, M.A. The effects of salt stress on growth, nitrate reduction and praline and glycinebetaine accumulation in Prosopis alba. - Braz. J. Plant Physiol. 16: 39-46, 2004. Go to original source...
    13. Mittler, R.: Oxidative stress, antioxidants and stress tolerance. - Trends Plant Sci. 7: 405-410, 2002. Go to original source...
    14. Murgia, I., Tarantino, D., Vannini, C., Bracale, M., Carravieri, S., Soave, C.: Arabidopsis thaliana plants over-expressing thylakoidal ascorbate peroxidase show increased resistance to paraquat-induced photo-oxidative stress and to nitric oxide-induced cell death. - Plant J. 38: 940-953, 2004. Go to original source...
    15. Mýtinová, Z., Motyka, V., Haisel, D., Gaudinová, A., Lubovská, Z., Wilhelmová, N.: Effect of abiotic stresses on the activity of antioxidative enzymes and contents of phytohormones in wild type and AtCKX2 transgenic tobacco plants. - Biol. Plant. 54: 461-470, 2010. Go to original source...
    16. Qu, C.X., Shen, S.D., Wang, X.F., Cui, Y.H., Song, W.P.: [Method research of measuring soluble protein contents of plant rough extraction using Coomassie brilliant blue.] - J. Suzhou Univ. 22: 82-85, 2006. [In Chin.]
    17. Reddy, A.R., Chaitanya, K.V., Vivekanandan, M.: Droughtinduced responses of photosynthesis and antioxidant metabolism in higher plant. - J. Plant Physiol. 161: 1189-1202, 2004. Go to original source...
    18. Robinson S.P., Downton W.J., Millhouse J.A.: Photosynthesis and ion content of leaves and isolated chloroplasts of salt stressed spinach. - Plant Physiol. 73: 238-242, 1983. Go to original source...
    19. Sato, Y., Masuta, Y., Saito, K., Murayama, S., Ozawa, K.: Enhanced chilling tolerance at the booting stage in rice by transgenic overexpression of the ascorbate peroxidase gene, OsAPXa. - Plant Cell Rep. 30: 399-406, 2011. Go to original source...
    20. Saxena, S.C., Joshi, P.K., Grimm, B., Arora, S.: Alleviation of ultraviolet-C-induced oxidative damage through overexpression of cytosolic ascorbate peroxidase. - Biologia 66: 1052-1059, 2012. Go to original source...
    21. Shigeoka, S., Ishikawa, T., Tamoi, M., Miyagawa, Y., Takeda, T., Yabuta, Y., Yoshimura, K.: Regulation and function of ascorbate peroxidase isoenzymes. - J. exp. Bot. 53:1305-1309, 2002. Go to original source...
    22. Sun, W.H., Duan, M., Shu, D.F., Yang, S., Meng, Q.W.: Overexpression of StAPX in tobacco improves seed germination and increases early seedling tolerance to salinity and osmotic stresses. - Plant Cell Rep. 29: 917-926, 2010. Go to original source...
    23. Vats, S.K., Kumar, S., Ahuja, P.S.: CO2 sequestration in plants: lesson from divergent strategies. - Photosynthetica 49: 481-496, 2011. Go to original source...
    24. Yabuta, Y., Motoki, T., Yoshimura, K., Takeda, T., Ishikawa, T., Shigeoka, S.: Thylakoid membrane-bound peroxidase is a limiting factor of antioxidative systems under photooxidative stress. - Plant J. 32: 915-925, 2002. Go to original source...
    25. Zhang, L.F., Ding, X.L.: [Establishment of a new lycopene determination method.] - Food Ferment. Ind. 27: 51-55, 2001. [In Chin.]
    26. Zhao, S.J., Shi, G.A., Dong, X.C.: [Laboratory Procedures in Plant Physiology.] - China Agriculture Science and Technology Press, Beijing 2002. [In Chin.]

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