biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:63-69, 2013 | DOI: 10.1007/s10535-012-0139-6

Molecular cloning and characterization of a chlorophyll degradation regulatory gene from bamboo

Y. -X. Chen1,2, Q. Wei1, B. -K. Kuai3, Y. -L. Ding1,*
1 Institute of Bamboo Research, Nanjing Forestry University, Nanjing, China
2 Nanjing Forest Police College, Nanjing, China
3 School of Life Sciences, Fudan University, Shanghai, China

Leaf senescence constituted the final stage of leaf development and it is always accompanied by the leaf yellowing. The non-yellowing gene (NYE1), initially identified from Arabidopsis in our laboratory, is a key regulatory gene responsible for chlorophyll degradation during senescence. In this study, an orthologue of AtNYE1 was isolated from the bamboo (Bambusa emeiensis cv. Viridiflavus) and tentatively named BeNYE1. The full length sequence of 1 386 bp contains an open reading frame of 801 bp. The protein encoded by BeNYE1 consists of 266 amino acids. Sequence analysis revealed that BeNYE1 had high similarity with other NYE/SGR proteins from various monocotyledon species. BeNYE1 was strongly induced by natural senescence and dark-induced senescence in bamboo. Driven by a 1.5 kb upstream fragment of AtNYE1, BeNYE1 could rescue the stay-green phenotype of nye1-1. The constitutive over-expression of BeNYE1 could accelerate the chlorophyll degradation. These results indicated that BeNYE1 might play an important role in the regulation of chlorophyll degradation during leaf senescence in bamboo.

Keywords: Bambusa emeiensis; leaf senescence; NYE1; stay-green phenotype
Subjects: molecular cloning; chlorophyll degradation regulatory gene; chlorophyll degradation; chlorophyll; bamboo; leaf senescence; stay-green phenotype

Received: August 18, 2011; Accepted: January 11, 2012; Published: March 1, 2013  Show citation

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Chen, Y.-X., Wei, Q., Kuai, B.-K., & Ding, Y.-L. (2013). Molecular cloning and characterization of a chlorophyll degradation regulatory gene from bamboo. Biologia plantarum, 57(1), 63-69. doi: 10.1007/s10535-012-0139-6
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