biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:133-138, 2013 | DOI: 10.1007/s10535-012-0131-1

Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea

J. Horáček1, L. ©vábová1, P. ©arhanová2, A. Lebeda2,*
1 Biotechnology Department, Agritec Plant Research Ltd., ©umperk, Czech Republic
2 Faculty of Science, Department of Botany, Palacký University in Olomouc, Olomouc-Holice, Czech Republic

Pea (Pisum sativum L.) somaclones of cultivars Adept, Komet and Bohatýr were obtained after selection in vitro with Fusarium solani filtrate and fusaric acid (FA). R2 regenerants were analysed by random amplification of polymorphic DNA (RAPD; OPAB4, P-14, UBC-556) and inter-retrotransposon amplification polymorphism (IRAP; Ogre) markers. Marker UBC-556 showed different banding patterns for each cultivar, but without specific bands for selected and control plants. Markers OPAB4, P14 and Ogre were useful for clear discrimination between selected and non-selected variants of all three cultivars. Flow cytometry analysis proved the same genome size of selected and non-selected pea lines. Therefore in vitro selection by pathogen derived agents could be the efficient method for obtaining of pea somaclones with increased resistance to F. solani.

Keywords: in vitro culture; fusarium wilt; IRAP; molecular markers; Pisum sativum; RAPD; selection agent
Subjects: in vitro culture; fusarium wilt; inter-retrotransposon amplification polymorphism; resistance to pathogen; molecular markers; pea

Received: November 2, 2011; Accepted: February 22, 2012; Published: March 1, 2013  Show citation

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Horáček, J., ©vábová, L., ©arhanová, P., & Lebeda, A. (2013). Variability for resistance to Fusarium solani culture filtrate and fusaric acid among somaclones in pea. Biologia plantarum, 57(1), 133-138. doi: 10.1007/s10535-012-0131-1
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