biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:237-246, 2012 | DOI: 10.1007/s10535-012-0082-6

Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene

Y. D. Liu1, Z. J. Yin2, J. W. Yu2, J. LI3, H. L. Wei2, X. L. Han1, F. F. Shen1,*
1 College of Agronomy, Shandong Agricultural University, Taian, Shandong, P.R. China
2 Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang, Henan, P.R. China
3 College of Pharmacy, Nankai University, Tianjin, P.R. China

The manipulation of cytokinin contents via Agrobacterium-mediated transformation is an efficient tool for delaying leaf senescence and improving the resistance to environmental stresses. In the present study, cotton transformants harbouring the Agrobacterium tumefaciens isopentenyl transferase (IPT) gene under the control of the promoter of Gossypium hirsutum cysteine proteinase (Ghcysp) were generated. PCR and Southern blot analysis indicated that the foreign DNA fragment was successfully integrated into the cotton genome. The chlorophyll and cytokinin contents, and ROS-scavenging enzymatic activities were significantly increased in transgenic cotton lines, which resulted in a significant delay in leaf senescence. The growth characteristics of transgenic cotton lines resembled the non-transgenic lines except delaying premature senescence and the lint yield and fiber quality of transgenic lines were improved. In addition, the transgenic lines had higher biomasses, IPT transcripts, and endogenous cytokinin contents compared with those of non-transgenic lines under 200 mM NaCl stress.

Keywords: Gossypium hirsutum; isopentenyl transferase; cytokinins; NaCl; senescence-specific promoter
Subjects: isopentenyl transferase; cytokinins; NaCl; senescence-specific promoter; leaf senescence; transgenic plants; salt tolerance; PCR; nucleotide sequence; gene construct; Southern blot; chlorophyll; gene expression; Northern blot

Received: March 4, 2010; Accepted: April 4, 2011; Published: June 1, 2012  Show citation

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Liu, Y.D., Yin, Z.J., Yu, J.W., LI, J., Wei, H.L., Han, X.L., & Shen, F.F. (2012). Improved salt tolerance and delayed leaf senescence in transgenic cotton expressing the Agrobacterium IPT gene. Biologia plantarum, 56(2), 237-246. doi: 10.1007/s10535-012-0082-6
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