biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:663-669, 2009 | DOI: 10.1007/s10535-009-0120-1

Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress

M. -H. Luo1,2, S. Yuan1, Y. -E. Chen1, W. -J. Liu1, J. -B. Du1, T. Lei1, M. -B. Wang1, H. -H. Lin1,*
1 Key Laboratory of Bio-Resources and Eco-Environment, Ministry of Education, College of Life Science, Sichuan University, Chengdu, P.R. China
2 Department of Life Science and Technology, Mianyang Normal University, Mianyang, P.R. China

The effects of exogenous salicylic acid (SA) on photosystem 2 (PS 2) in barley (Hordeum vulgare L.) seedlings were investigated. SA pretreatment provided protection against subsequent osmotic stress. The highest protective effect of 0.25 mM SA was confirmed by determination of chlorophyll fluorescence, electrolyte leakage, malonyldialdehyde contents, PS 2 mRNAs and proteins. SA pretreatment increased reactive oxygen species (ROS), decreased net photosynthetic rate and stomatal conductance immediately, but prevented ROS accumulation during subsequent osmotic stress by activating antioxidant enzymes. Elimination of H2O2 during SA pretreatment inhibited almost all above mentioned SA effects. Therefore, SA pretreatment enhanced osmotic stress tolerance in barley seedlings mainly through ROS signals, rather than SA itself. The only SA-dependent and ROS-independent effect of exogenous SA on PS 2 was reduction of non-photochemical quenching.

Keywords: chlorophyll fluorescence; electrolyte leakage; Hordeum vulgare; net photosynthetic rate; reactive oxygen species; stomatal conductance
Subjects: barley; chlorophyll fluorescence; chloroplast; electrolyte leakage; Hordeum vulgare; hydrogen peroxide; malondialdehyde; Northern blot analyses; osmotic stress; photosynthetic rate; photosystems; polyethyloene glycol; proteins; relative water content (RWC); salicylic acid; stomatal conductance

Received: November 14, 2007; Accepted: May 25, 2008; Published: December 1, 2009  Show citation

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Luo, M.-H., Yuan, S., Chen, Y.-E., Liu, W.-J., Du, J.-B., Lei, T., Wang, M.-B., & Lin, H.-H. (2009). Effects of salicylic acid on the photosystem 2 of barley seedlings under osmotic stress. Biologia plantarum, 53(4), 663-669. doi: 10.1007/s10535-009-0120-1
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