biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:530-538, 2009 | DOI: 10.1007/s10535-009-0096-x

Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots

M. Kotis1, T. A. Yupsanis2,*, T. D. Syros1,2, A. S. Economou1
1 Department of Horticulture, School of Agriculture, Aristotle University, Thessaloniki, Greece
2 Department of Biochemistry, School of Chemistry, Aristotle University, Thessaloniki, Greece

Specific activities and isoform patterns of peroxidases, acid phosphatases, DNases and RNases were studied in relation to in vitro rooting of Petunia × hybrida microshoots in the presence of 4 µM indole-3-butyric acid (IBA). Specific activities of the above enzymes increased in the course of rooting. Rhizogenesis could be related with an increased specific activity of peroxidases during the initiation phase, in parallel with increased lignin content. Twelve peroxidases, six anionic (A1-A6) and six cationic (C1-C6), seven acid phosphatases (ACP1-ACP7), seven RNases (R1-R7) and four DNases (D1-D4) isoforms were detected following native PAGE. Variation in the number of the above isoforms and their quantity was observed during different stages of rooting. Particularly, A2, A3, C3, C4, C5, ACP2, R1, R2, R3, and D4 isoforms appeared after the induction phase and could be related to emergence of root primordia. Additionally, R3 and D4 could be associated with cell division and differentiation, since these are only expressed in rooted microshoots. Moreover, the higher number of roots in IBA-treated microshoots could be related to the higher expression of RNase and DNase isoforms during initiation and expression phases.

Keywords: electrophoresis; IBA; lignin; microcuttings; nucleases; vegetative propagation
Subjects: acid phosphatase; auxins; in vitro culture, rooting; microcuttings; peroxidase; petunia; Petunia hybrida; ribonuclease

Received: January 19, 2007; Accepted: March 15, 2008; Published: September 1, 2009  Show citation

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Kotis, M., Yupsanis, T.A., Syros, T.D., & Economou, A.S. (2009). Peroxidase, acid phosphatase, RNase and DNase activity and isoform patterns during in vitro rooting of Petunia × hybrida microshoots. Biologia plantarum, 53(3), 530-538. doi: 10.1007/s10535-009-0096-x
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