biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:271-277, 2009 | DOI: 10.1007/s10535-009-0050-y

A new tip homolog, ShTIP, from Salicornia shows a different involvement in salt stress compared to that of TIP from Arabidopsis

N. Ermawati1, Y. S. Liang1, J.-Y. Cha1, D. Shin1, M. H. Jung1, J. J. Lee2, B.-H. Lee1, C.-D. Han1, K. H. Lee1, D. Son1,2,*
1 Division of Applied Life Science (BK21), Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju, Korea
2 Department of Applied Biology and Environmental Science, Gyeongsang National University, Jinju, Korea

To obtain an insight into the comprehensive molecular characteristics of the salt tolerance mechanism, we performed a screening for salt inducible genes in a halophytic plant, Salicornia herbacea, using mRNA differential display. A comparative analysis of gene expression in Salicornia grown in control and salt-stressed conditions led to the detection of a gene that was induced by salt. Both sequence analysis and a subsequent database search revealed that this gene was highly homologous to tonoplast intrinsic proteins (TIPs) from a variety of plant species. This gene, designated as ShTIP, is 1014 bp in size and contains a coding region of 762 nucleotides, which encodes a protein of 254 amino acids. Northern blot analysis revealed that ShTIP was predominantly expressed in shoots under normal conditions. However, salt stress induced high expression of ShTIP in both the shoots and roots. The expression of ShTIP in a salt-sensitive calcineurin-deficient yeast mutant (cnbΔ) resulted in a resistance to the high salt conditions. In addition, we compared the expression of a TIP gene in Arabidopsis with that of ShTIP under different conditions and found that the Salicornia TIP has a different regulatory mechanism for adapting to salt stress conditions compared with the glycophyte Arabidopsis TIP. These results indicate that ShTIP plays an important role in salt tolerance.

Keywords: halophyte; glycophyte; Salicornia herbacea; salinity; tonoplast intrinsic protein
Subjects: abscisic acid (ABA); amino acids; aquaporins; Arabidopsis thaliana; gene expression; polyethyloene glycol; Salicornia herbacea; salinity; tonoplast intrinsic proteins (TIP)

Received: May 17, 2007; Accepted: January 9, 2008; Published: June 1, 2009  Show citation

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Ermawati, N., Liang, Y.S., Cha, J.-Y., Shin, D., Jung, M.H., Lee, J.J., ... Son, D. (2009). A new tip homolog, ShTIP, from Salicornia shows a different involvement in salt stress compared to that of TIP from Arabidopsis. Biologia plantarum, 53(2), 271-277. doi: 10.1007/s10535-009-0050-y
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