Biologia plantarum 52:597-600, 2008 | DOI: 10.1007/s10535-008-0119-z
Promotion of direct somatic embryogenesis of Oncidium by adjusting carbon sources
- 1 Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, Republic of China
- 2 Institute of Biotechnology, National University of Kaohsiung, Kaohsiung 811, Taiwan, Republic of China
To further optimize a culture medium for induction of direct embryo formation of Oncidium cvs. Gower Ramsey and Sweet Sugar, five kinds of carbon sources, cellibiose, fructose, glucose, maltose and sucrose at 10, 20, 30 and 60 g dm-3 were tested in this study. Cellibiose supply had an inhibitory effect and resulted in high percentage of explant browning in both cultivars. By contrast, fructose, glucose and sucrose were all effective for direct embryo induction. In cv. Gower Ramsey, the suitable ranges of concentration were found at 30-60 g dm-3 of sucrose, 10-20 g dm-3 of glucose and 20-30 g dm-3 of fructose, respectively. The suitable ranges for cv. Sweet Sugar were at 20-60 g dm-3 of sucrose, 10-30 g dm-3 of glucose, 10-20 g dm-3 of fructose and 30-60 g dm-3 of maltose, respectively. The highest amount of embryos was obtained at 30 g dm-3 of sucrose for cv. Gower Ramsey and at 20 g dm-3 of glucose for cv. Sweet Sugar.
Keywords: embryogenic response; leaf explant; orchid
Subjects: cultivar and genotype differences; in vitro culture, cell suspension; in vitro culture, somatic embryogenesis; Oncidium; sugars
Received: May 24, 2007; Accepted: May 2, 2008; Published: September 1, 2008 Show citation
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