biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 52:259-266, 2008 | DOI: 10.1007/s10535-008-0056-x

Molecular mapping of genomic regions associated with wheat seedling growth under osmotic stress

S. Landjeva1,2,*, K. Neumann1, U. Lohwasser1, A. Börner1
1 Leibniz Institute of Plant Genetics and Crop Research, Gatersleben, Germany
2 Institute of Genetics, Bulgarian Academy of Sciences, Sofia, Bulgaria

A quantitative trait loci (QTL) approach was applied to dissect the genetic control of the common wheat seedling response to osmotic stress. A set of 114 recombinant inbred lines was subjected to osmotic stress from the onset of germination to the 8th day of seedling development, induced by the presence of 12 % polyethylene glycol. Root, coleoptile and shoot length, and root/shoot length ratio were compared under stress and control conditions. In all, 35 QTL mapping to ten chromosomes, were identified. Sixteen QTL were detected in controls, 17 under stressed conditions, and two tolerance index QTL were determined. The majority of the QTL were not stress-specific. In regions on five chromosome arms (1AS, 1BL, 2DS, 5BL and 6BL) the QTL identified under stress co-mapped with QTL affecting the same trait in controls, and these were classified as seedling vigour QTL, in addition to those expressed in controls. Tolerance-related QTL were detected on four chromosome arms. A broad region on chromosome 1AL, including five QTL, with a major impact of the gene Glu-A1 (LOD 3.93) and marker locus Xksuh9d (LOD 2.91), positively affected root length under stress and tolerance index for root length, respectively. A major QTL (LOD 3.60), associated with marker locus Xcdo456a (distal part of chromosome arm 2BS) determined a tolerance index for shoot length. Three minor QTL (LOD < 3.0) for root length and root/shoot length ratio under osmotic stress were identified in the distal parts of chromosome arms 6DL (marker locus Xksud27a) and 7DL (marker locus Xksue3b). Selecting for the favourable alleles at marker loci associated with the detected QTL for growth traits may represent an efficient approach to enhance the plants' ability to maintain the growth of roots, coleoptile and shoots in drought-prone soils at the critical early developmental stages.

Keywords: drought; germination; ITMI; polyethylene glycol; quantitative trait loci; tolerance index; Triticum aestivum
Subjects: chromosomes; coleoptile; germination; osmotic potential; Triticum aestivum; water stress; wheat

Received: July 9, 2006; Accepted: January 23, 2007; Published: June 1, 2008  Show citation

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Landjeva, S., Neumann, K., Lohwasser, U., & Börner, A. (2008). Molecular mapping of genomic regions associated with wheat seedling growth under osmotic stress. Biologia plantarum, 52(2), 259-266. doi: 10.1007/s10535-008-0056-x
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