biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:764-768, 2007 | DOI: 10.1007/s10535-007-0156-z

Analysis of genetic diversity among Swertia chirayita genotypes

P. Joshi1,*, V. Dhawan1
1 TERI University, New Delhi, India

Inter simple sequence repeat (ISSR) markers were used to analyse genetic diversity of Swertia chirayita genotypes collected from the temperate Himalayas of India. Allied species of Swertia chirayita were used in the study as outliers. Nineteen UBC primers generated a total of 315 ISSR bands, revealing 98.7 % polymorphism among the genotypes assayed. This was reduced to 42.5 % when the outliers were excluded. The results revealed a high genetic diversity within the genotypes.

Keywords: biodiversity; inter simple sequence repeat markers; medicinal plants
Subjects: genetic diversity, biodiversity; medicinal plants; polymerase chain reaction (PCR); Swertia chirayita

Received: October 4, 2005; Accepted: June 2, 2006; Published: December 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Joshi, P., & Dhawan, V. (2007). Analysis of genetic diversity among Swertia chirayita genotypes. Biologia plantarum, 51(4), 764-768. doi: 10.1007/s10535-007-0156-z
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bhatia, A., Karan, M., Vasisht, K.: Morphological and chemotypic comparison of certain Indian species of Swertia.-J. med. aromat. Plant Sci. 25: 336-343, 2003.
    2. Blair, M., Panaud, W.O., McCouch, S.R.: Inter simple sequence repeat amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza savita L.).-Theor appl Genet 98: 780-794, 1999. Go to original source...
    3. Dangi, SR., Lagu, M.D., Chaudhury, L.B., Ranjekar, P.K., Gupta, V.S.: Asessment of genetic diversity in Trigonella foecum-graecum and Trigonella caerulea using ISSR and RAPD markers.-BMC Plant Biol. 4: 13-24, 2004. Go to original source...
    4. Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissue.-Focus 12: 13-15, 1990. Go to original source...
    5. Frediani, M., Caputo, P.: Phylogenetic relationships among annual and perennial species of genus Cicer as inferred from ITS sequences of nuclear ribosomal DNA.-Biol. Plant. 49: 47-52, 2005 Go to original source...
    6. Gilbert, J.E., Lewis, R.V., Wilkinson, M.J., Caligari, P.D.S.: Developing an appropriate strategy to assess genetic variability in plant germplasm collections.-Theor. appl. Genet. 98: 1125-1131, 1999. Go to original source...
    7. Jaccard, P.: Nouvelles reserches sur la distribution flora.-Bull. Soc. Vaudoise Sci. nat. 44: 223-270, 1908.
    8. Karan, M., Vasisht, K., Handa, S.S.: Morphological and chromatographic comparison of certain Indian species of Swertia.-J. med. aromat. Plant Sci. 19: 955-963, 1997.
    9. Khoshoo, T.N., Tandon, S.R.: Cytological, morphological and pollination studies of some Himalayan species of Swertia.-Caryologia (Pisa) 16: 445-477, 1963.
    10. McGregor, C.E., Lambert, C.A., Greyling, M.M., Louw, J.H., Warnich, L.A.: Comparative assessment of DNA fingerprinting techniques (RAPD, ISSR, AFLP and SSR) in tetraploid potato (Solanum tuberosum L.).-Euphytica 113: 135-144, 2000. Go to original source...
    11. Nagaoka, T., Ogihara, Y.: Applicability of inter-simple sequence repeat markers in wheat for use as DNA markers in comparison to RFLP and RAPD markers.-Theor. appl. Genet. 97: 597-602, 1997. Go to original source...
    12. Powell, W., Morgante, R., Andre, C., Hanafey, M., Vogel, J., Tingey, S., Rafalsky, A.: The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis.-Mol. Breed. 2: 225-238, 1996. Go to original source...
    13. Rao, V.R., Hodgkin, T.: Genetic diversity and conservation and utilisation of plant genetic resources.-Plant Cell Tissue Organ Cult. 68: 1-19, 2002.
    14. Rohlf, F.J.: NT SYS-pc Numerical taxonomy and multivariate analysis system, Version 2.0.-Applied Biostatistics. New York 1998.
    15. Ruas, P. M., Ruas, C.F., Rampin, L., Carvalho, V.P., Ruas, E.A., Sera, T.: Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter Simple Sequence Repeat) markers.-Genet. mol. Biol. 26: 319-327, 2003. Go to original source...
    16. Shanker, R.U., Ganeshaiah, K.N.: Mapping genetic diversity of Phyllanthus emblica: Forest gene banks as a new approach for in situ conservation of genetic resources.-Curr. Sci. 73: 163-168, 1997.
    17. Verma, N.K., Kumar, A.: Isozyme polymorphism and genetic diversity among Swertia species-endangered medicinal plants of Himalayas.-Indian J. Plant Genet. Resour. 14: 74-77, 2001.
    18. Weising, J., Nybom, H., Wolff, K., Meyer, W.: DNA fingerprinting in plants and fungi.-CRC Press, Boca Raton, 1995.
    19. Wu, C., Cheng, Z., Huang, X., Yin, S., Cao, K., Sun, C.: Genetic diversity among and within populations of Oryza granulata from Yunnan of China revealed by RAPD and ISSR markers: implications for conservation of the endangered species.-Plant Sci. 167: 35-42, 2004. Go to original source...
    20. Yap, I.V., Nelson, R.J.: WINBOOT, a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA based dendrograms.-IRRI Discussion Paper Ser. No. 14. International Rice Research Institute, Los Baños 1996.
    21. Zhang, H.Y., Liu, X.Z., He, C.S., Zheng, C.M.: Random amplified DNA polymorphism of Nicotiana tabacum L. cultivars.-Biol. Plant. 49: 605-607, 2005. Go to original source...

    Return to the content