biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 51:34-42, 2007 | DOI: 10.1007/s10535-007-0008-x

Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo

Z. A. Aziz1, M. R. Davey1,*, J. B. Power1, P. Anthony1, R. M. Smith2, K. C. Lowe3
1 Plant Sciences Division, School of Biosciences, University of Nottingham, Loughborough, UK
2 Department of Chemistry, Loughborough University, Loughborough, UK
3 School of Biology, University of Nottingham, University Park, Nottingham, UK

The localization was determined of the triterpenoids, asiaticoside and madecassoside, in different organs of glasshouse-grown plants and cultured material, including transformed roots, of two phenotypes of Centella asiatica (L.) Urban of Malaysian origin. Methanolic extracts of asiaticoside and madecassoside were prepared for gradient HPLC analysis. The two phenotypes of C. asiatica exhibited differences in terpenoid content that were tissue specific and varied between glasshouse-grown plants and tissue culture-derived material. Terpenoid content was highest in leaves, with asiaticoside (0.79 ± 0.03 and 1.15 ± 0.10 % of dry mass) and madecassoside [0.97 ± 0.06 and 1.65 ± 0.01 %(d.m.)] in the fringed (F) and smooth leaf (S) phenotypes, respectively. Roots of the F-phenotype contained the lowest content of asiaticoside [0.12 ± 0.01 %(d.m.)], whereas petioles of S-phenotype plants contained the lowest content of asiaticoside [0.16 ± 0.01 %(d.m.)] and madecassoside [0.18 ± 0.14 %(d.m.)]. Transformed roots were induced using Agrobacterium rhizogens and their growth was maximal on Murashige and Skoog basal medium supplemented with 60 g dm-3 sucrose. However, asiaticoside and madecassoside were undetectable in transformed roots and undifferentiated callus.

Keywords: Agrobacterium transformation; phenotypic variation; terpenoids
Subjects: Agrobacterium rhizogenes; asiaticoside; auxins; Centella asiatica; cytokinins; ex vitro transfer; in vitro culture, callus growth and duration; in vitro culture, regeneration, proliferation, differentiation; in vitro culture, root cultivation, rooting; medicinal plants; nutrient medium, Murashige and Skoog (MS); sucrose; transgenic plants; triterpenes, triterpenoids

Received: March 8, 2005; Accepted: January 17, 2006; Published: March 1, 2007  Show citation

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Aziz, Z.A., Davey, M.R., Power, J.B., Anthony, P., Smith, R.M., & Lowe, K.C. (2007). Production of asiaticoside and madecassoside in Centella asiatica in vitro and in vivo. Biologia plantarum, 51(1), 34-42. doi: 10.1007/s10535-007-0008-x
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