biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 49:427-430, 2005 | DOI: 10.1007/s10535-005-0021-x

Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger

S. Uranbey1,*
1 Ministry of Agriculture and Rural Affairs, Central Research Institute for Field Crops, Ankara, Turkey

A high frequency adventitious shoot regeneration protocol was developed for henbane (Hyoscyamus niger L.) using thidiazuron (TDZ). Hypocotyl, cotyledon and stem explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of N6-benzylaminopurine and TDZ. MS medium supplemented with 16 μM TDZ was the most effective for providing 100 % regeneration frequency associated with a 19.53 shoots per hypocotyl explant. Plantlets were rooted on MS medium supplemented with different concentrations of indole-3-butyric acid (IBA) and α-naphthaleneacetic acid. High rooting and survival was achieved using half strength MS medium supplemented with 8 μM IBA.

Keywords: N6-benzylaminopurine; henbane; indole-3-butyric acid; α-naphthaleneacetic acid; rooting; tissue culture
Subjects: adventitious shoot, root; auxins; cytokinins; henbane, shoot regeneration; Hyoscyamus niger; in vitro culture, adventitious shoots and roots; in vitro culture, callus induction; in vitro culture, regeneration, organogenesis, morphogenesis; medicinal plants; rooting; thidiazuron (TDZ)

Received: December 2, 2003; Accepted: November 23, 2004; Published: September 1, 2005  Show citation

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Uranbey, S. (2005). Thidiazuron induced adventitious shoot regeneration in Hyoscyamus niger. Biologia plantarum, 49(3), 427-430. doi: 10.1007/s10535-005-0021-x
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