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Synthesis and characterization of zinc derivatized 3, 5-dihydroxy 4′, 7-dimethoxyflavone and its anti leishmaniasis activity against Leishmania donovani

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Abstract

This study reports the synthesis and characterization of zinc derivatized 3,5-dihydroxy 4′, 7- dimethoxyflavone (DHDM-Zn) compound for the development of new antileishmanial agents. The interaction studies of DHDM with zinc were carried out by UV spectra and fluorescence spectra analysis. Characterization of the complex was further accomplished by multi-spectroscopic techniques such as FTIR, Raman, HRMS, NMR, FESEM-EDX. The morphological and topographical studies of synthesized DHDM-Zn were carried out using FESEM with EDX. Further, it was demonstrated that DHDM-Zn exhibited an excellent in vitro antagonistic effect against the promastigote form of L. donovani. In addition, the possible mechanisms of promastigote L. donovani cell death, by involvement of derivatized compound in arrest of the cell cycle in the G1 phase and residual cell count reduction were investigated. Promastigote growth kinetics performed in the presence of the derivatized compound revealed a slow growth rate. The combination of growth kinetics and cell cycle analysis, made it possible to interpret and classify the cause of leishmanial cell death accurately. These results support that zinc derivatized complex (DHDM-Zn) might work as a lead compound for designing and developing a new antileishmanial drug.

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Acknowledgements

MKG thanks MHRD for student fellowship. The authors would like to thank, Department of Biosciences and Bioengineering (BSBE) and Central Instrument Facility (CIF) of IIT Guwahati for instrumentation facility.

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This study did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Applied Biodiversity Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, North Guwahati, India

    Manish Kumar Gupta & Latha Rangan

  2. Protein Biochemistry and Molecular Parasitology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Assam, 781039, Guwahati, India

    Adarsh Kumar Chiranjivi

  3. Centre for the Environment, Indian Institute of Technology Guwahati, Assam, 781039, Guwahati, India

    Tanmay Dutta

  4. School of Biochemical Engineering, Indian Institute of Technology (BHU) Varanasi, 221005, Varanasi, India

    Vikash Kumar Dubey

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  1. Manish Kumar Gupta
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Correspondence to Vikash Kumar Dubey or Latha Rangan.

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Gupta, M.K., Chiranjivi, A.K., Dutta, T. et al. Synthesis and characterization of zinc derivatized 3, 5-dihydroxy 4′, 7-dimethoxyflavone and its anti leishmaniasis activity against Leishmania donovani. Biometals 35, 285–301 (2022). https://doi.org/10.1007/s10534-022-00364-x

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