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Aluminium stress disrupts metabolic performance of Plantago almogravensis plantlets transiently

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Abstract

Little is known about how tolerant plants cope with internalized aluminium (Al). Tolerant plants are known to deploy efficient detoxification mechanisms, however it is not known to what extent the primary and secondary metabolism is affected by Al. The aim of this work was to study the metabolic repercussions of Al stress in the tolerant plant Plantago almogravensis. P. almogravensis is well adapted to acid soils where high concentrations of free Al are found and has been classified as a hyperaccumulator. In vitro reared plantlets were used for this purpose in order to control Al exposure rigorously. The metabolome of P. almogravensis plantlets as well as its metabolic response to the supply of sucrose was characterized. The supply of sucrose leads to an accumulation of amino acids and secondary metabolites and consumption of carbohydrates that result from increased metabolic activity. In Al-treated plantlets the synthesis of amino acids and secondary metabolites is transiently impaired, suggesting that P. almogravensis is able to recover from the Al treatment within the duration of the trials. In the presence of Al the consumption of carbohydrate resources is accelerated. The content of some metabolic stress markers also demonstrates that P. almogravensis is highly adapted to Al stress.

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Acknowledgments

The authors acknowledge support from the Portuguese Foundation for Science and Technology (FCT, Project PTDC/AGR-AAM/102664/2008 and the post-doc grant assigned to T. Grevenstuk SFRH/BPD/73293/2010) and thank Daniel Jacob for administrating MeRy-B repository and BioStatFlow.

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Authors and Affiliations

  1. MeditBio, Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Tomás Grevenstuk & Anabela Romano

  2. INRA, UMR1332 Biologie du Fruit & Pathologie, Centre INRA de Bordeaux, 33140, Villenave d’ Ornon, France

    Annick Moing & Catherine Deborde

  3. UMR1332 Biologie du Fruit & Pathologie, Centre INRA de Bordeaux, Univ Bordeaux, 33140, Villenave d’Ornon, France

    Mickaël Maucourt

  4. Metabolome Facility of Bordeaux Functional Genomics Centre, MetaboHUB, Centre INRA de Bordeauxb, IBVM, 33140, Villenave d’Ornon, France

    Mickaël Maucourt

Authors
  1. Tomás Grevenstuk
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  2. Annick Moing
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  3. Mickaël Maucourt
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  4. Catherine Deborde
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  5. Anabela Romano
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Correspondence to Anabela Romano.

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Grevenstuk, T., Moing, A., Maucourt, M. et al. Aluminium stress disrupts metabolic performance of Plantago almogravensis plantlets transiently. Biometals 28, 997–1007 (2015). https://doi.org/10.1007/s10534-015-9884-2

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  • DOI: https://doi.org/10.1007/s10534-015-9884-2

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