Abstract
The autotrophic process for nitrogen removal has attracted worldwide attention in the field of wastewater treatment, and the performance of this process is greatly influenced by the size of granular sludge particles present in the system. In this work, the granular sludge was divided into three groups, i.e. large size (> 1.2 mm), medium size (0.6–1.2 mm) and small size (< 0.6 mm). The medium granular sludge was observed to dominate at high volumetric nitrogen loading rates, while offering strong support for good performance. Its indispensable contribution was found to originate from improved settling velocity (0.84 ± 0.10 cm/s), high SOUR-A (specific oxygen uptake rate for ammonia oxidizing bacteria, 25.93 mg O2/g MLVSS/h), low SOUR-N (specific oxygen uptake rate for nitrite oxidizing bacteria, 3.39 mg O2/g MLVSS/h), and a reasonable microbial spatial distribution.
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References
Amann RI (1995) In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In: Akkermans ADL, van Elsas JD, de Bruijn FJ (eds) Molecular microbial ecology manual. Springer, New York, pp 331–345
APHA-AWWA-WEF (2012) Standard methods for the examination of water and wastewater, 22nd edn. American Public Health Association, Washington, DC
Bouwman AM, Bosma JC, Vonk P, Wesselingh JHA, Frijlink HW (2004) Which shape factor(s) best describe granules? Powder Technol 146:66–72
Bürgmann H, Jenni S, Vazquez F, Udert KM (2011) Regime shift and microbial dynamics in a sequencing batch reactor for nitrification and anammox treatment of urine. Appl Environ Microb 77:5897–5907
Chen J, Zheng P, Yu Y, Mahmood Q, Tang C (2010) Enrichment of high activity nitrifers to enhance partial nitrification process. Bioresour Technol 101:7293–7298
Ergas SJ, Aponte-Morales V (2014) 3.8—biological nitrogen removal. In: Ahuja S (ed) Comprehensive water quality and purification. Elsevier, Waltham, pp 123–149
Fux C, Siegrist H (2004) Nitrogen removal from sludge digester liquids by nitrification/denitrification or partial nitritation/anammox: environmental and economical considerations. Water Sci Technol 50:19–26
Ganguly U (1990) On the prediction of terminal settling velocity of solids in liquid–solid systems. Int J Miner Process 29:235–247
Gao DW, Huang XL, Tao Y, Cong Y, Wang XL (2015) Sewage treatment by an UAFB–EGSB biosystem with energy recovery and autotrophic nitrogen removal under different temperatures. Bioresour Technol 181:26–31
Ge S, Peng Y, Qiu S, Zhu A, Ren N (2014) Complete nitrogen removal from municipal wastewater via partial nitrification by appropriately alternating anoxic/aerobic conditions in a continuous plug-flow step feed process. Water Res 55:95–105
Guillén JS, Jayawardana L, Vazquez CL, de Oliveira Cruz L, Brdjanovic D, van Lier J (2015) Autotrophic nitrogen removal over nitrite in a sponge-bed trickling filter. Bioresour Technol 187:314–325
Guo C-Z, Fu W, Chen X-M, Peng D-C, Jin P-K (2013) Nitrogen-removal performance and community structure of nitrifying bacteria under different aeration modes in an oxidation ditch. Water Res 47:3845–3853
Hao X, Wang Q, Zhang X, Cao Y, Mark Loosdrecht CM (2009) Experimental evaluation of decrease in bacterial activity due to cell death and activity decay in activated sludge. Water Res 43:3604–3612
Hendrickx TL, Wang Y, Kampman C, Zeeman G, Temmink H, Buisman CJ (2012) Autotrophic nitrogen removal from low strength waste water at low temperature. Water Res 46:2187–2193
Jin R-c Hu, B-l Zheng P, Qaisar M, A-h Hu, Islam E (2008) Quantitative comparison of stability of ANAMMOX process in different reactor configurations. Bioresour Technol 99:1603–1609
Li S, Chen Y-P, Li C, Guo J-S, Fang F, Gao X (2012) Influence of free ammonia on completely autotrophic nitrogen removal over nitrite (CANON) process. Appl Biochem Biotechnol 167:694–704
Matsumoto S et al (2010) Microbial community structure in autotrophic nitrifying granules characterized by experimental and simulation analyses. Environ Microbiol 12:192–206
Mobarry BK, Wagner M, Urbain V, Rittmann BE, Stahl DA (1996) Phylogenetic probes for analyzing abundance and spatial organization of nitrifying bacteria. Appl Environ Microb 62:2156–2162
Mu Y, Ren T-T, Yu H-Q (2008) Drag coefficient of porous and permeable microbial granules. Environ Sci Technol 42:1718–1723
Park S, Bae W, Rittmann BE, Kim S, Chung J (2010) Operation of suspended-growth shortcut biological nitrogen removal (SSBNR) based on the minimum/maximum substrate concentration. Water Res 44:1419–1428
Rodriguez-Sanchez A et al (2017) Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chem Eng J 245:993–999
Rui D, Cao S, Wang S, Meng N, Peng Y (2016) Performance of partial denitrification (PD)-ANAMMOX process in simultaneously treating nitrate and low C/N domestic wastewater at low temperature. Bioresour Technol 219:420
Satoh H, Miura Y, Tsushima I, Okabe S (2007) Layered structure of bacterial and archaeal communities and their in situ activities in anaerobic granules. Appl Environ Microb 73:7300–7307
Schmid M et al (2003) Candidatus “Scalindua brodae”, sp. nov., Candidatus “Scalindua wagneri”, sp. nov., two new species of anaerobic ammonium oxidizing bacteria. Syst Appl Microbiol 26:529–538
Su KZ, Ni BJ, Yu HQ (2013) Modeling and optimization of granulation process of activated sludge in sequencing batch reactors. Biotechnol Bioeng 110:1312–1322
Tang CJ et al (2010) Enrichment features of anammox consortia from methanogenic granules loaded with high organic and methanol contents. Chemosphere 79:613–619
Vlaeminck SE et al (2010) Aggregate size and architecture determine microbial activity balance for one-stage partial nitritation and anammox. Appl Environ Microb 76:900–909
Volcke EIP, Picioreanu C, De Baets B, van Loosdrecht MCM (2010) Effect of granule size on autotrophic nitrogen removal in a granular sludge reactor. Environ Technol 31:1271–1280
Volcke E, Picioreanu C, De Baets B, van Loosdrecht M (2012) The granule size distribution in an anammox-based granular sludge reactor affects the conversion—implications for modeling. Biotechnol Bioeng 109:1629–1636
Wang L, Zheng P, Xing YJ, Li W, Yang J, Abbas G et al (2014) Effect of particle size on the performance of autotrophic nitrogen removal in the granular sludge bed reactor and microbiological mechanisms. Bioresour Technol 157:240
Winkler MKH, Kleerebezem R, Strous M, Chandran K, van Loosdrecht MCM (2013) Factors influencing the density of aerobic granular sludge. Appl Microbiol Biot 97:7459–7468
Xu D, Xiao E, Xu P, Lin L, Zhou Q, Xu D, Wu Z (2017) Bacterial community and nitrate removal by simultaneous heterotrophic and autotrophic denitrification in a bioelectrochemically-assisted constructed wetland. Bioresour Technol 245:993–999
Yao Z-B, Cai Q, Zhang D-J, Xiao P-Y, Lu P-L (2013) The enhancement of completely autotrophic nitrogen removal over nitrite (CANON) by N2H4 addition. Bioresour Technol 146:591–596
Zhang X, Li D, Liang Y, Zeng H, He Y, Zhang Y, Zhang J (2014) Performance and microbial community of completely autotrophic nitrogen removal over nitrite (CANON) process in two membrane bioreactors (MBR) fed with different substrate levels. Bioresour Technol 152:185–191
Zheng P, Lin FM, Hu BL, Chen JS (2004) Start-up of anaerobic ammonia oxidation bioreactor with nitrifying activated sludge. J Environ Sci 16:13–16
Acknowledgements
Financial supports of this work by National Natural Science Foundation of China (U1609214;51408570), Major Projects for Science and Technology Development of Zhejiang Province, China (2015C02037), Zhejiang Science and Technology Program key projects, China (2017C03010), and the Research Fund of Tianjin Key Laboratory of Aquatic Science and Technology are greatly appreciated.
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Ya-juan, X., Jun-yuan, J., Ping, Z. et al. The effect and biological mechanism of granular sludge size on performance of autotrophic nitrogen removal system. Biodegradation 29, 339–347 (2018). https://doi.org/10.1007/s10532-018-9836-y
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DOI: https://doi.org/10.1007/s10532-018-9836-y