Abstract
Metamifop is universally used in agriculture as a post-emergence aryloxyphenoxy propionate herbicide (AOPP), however its microbial degradation mechanism remains unclear. Consortium ME-1 isolated from AOPP-contaminated soil can degrade metamifop completely after 6 days and utilize it as the carbon source for bacterial growth. Meanwhile, consortium ME-1 possessed the ability to degrade metamifop stably under a wide range of pH (6.0–10.0) or temperature (20–42 °C). HPLC–MS analysis shows that N-(2-fluorophenyl)-2-(4-hydroxyphenoxy)-N-methyl propionamide, 2-(4-hydroxyphenoxy)-propionic acid, 6-chloro-2-benzoxazolinone and N-methyl-2-fluoroaniline, were detected and identified as four intermediate metabolites. Based on the metabolites identified, a putative metabolic pathway of metamifop was proposed for the first time. In addition, the consortium ME-1 was also able to transform or degrade other AOPP such as fenoxaprop-p-ethyl, clodinafop-propargyl, quizalofop-p-ethyl and cyhalofop-butyl. Moreover, the community structure of ME-1 with lower microbial diversity compared with the initial soil sample was investigated by high throughput sequencing. β-Proteobacteria and Sphingobacteria were the largest class with sequence percentages of 46.6% and 27.55% at the class level. In addition, 50 genera were classified in consortium ME-1, of which Methylobacillus, Sphingobacterium, Bordetella and Flavobacterium were the dominant genera with sequence percentages of 25.79, 25.61, 14.68 and 9.55%, respectively.
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Acknowledgements
This work was supported by the National Natural Science Foundation of China (Nos. 21390200 and 31560031), the Jiangsu Province Natural Science Foundation for Youths (No. BK20140940), China Postdoctoral Science Foundation (No. 2016M601787), and the Postdoctoral Foundation of Jiangsu Province (No. 1601043B), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, A Project Supported by Program for New Century Excellent Talents in University, Program for Changjiang Scholars and Innovative Research Team in University (No. 06-A-047).
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Dong, W., Liu, K., Wang, F. et al. The metabolic pathway of metamifop degradation by consortium ME-1 and its bacterial community structure. Biodegradation 28, 181–194 (2017). https://doi.org/10.1007/s10532-017-9787-8
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DOI: https://doi.org/10.1007/s10532-017-9787-8