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Development of a treatment solution for reductive dechlorination of hexachloro-1,3-butadiene in vadose zone soil

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Abstract

The biodegradation of chlorinated organics in vadose zone soils is challenging owing to the presence of oxygen, which inhibits reductive dehalogenation reactions and consequently the growth of dehalorespiring microbes. In addition, the hydraulic conductivity of vadose zone soils is typically high, hence attempts to remediate such zones with biostimulation solutions are often unsuccessful due to the short residence times for these solutions to act upon the native bacterial community. In this study we have identified sodium alginate as a hydrogel polymer that can be used to increase the residence time of a nutrient solution in an unsaturated sandy soil. Additionally we have identified neutral red as a redox active compound that can catalyse the reductive dechlorination of the chlorinated organic hexachloro-1,3-butadiene by activated sludge fed with lactate and acetate. Finally we have shown that a nutrient solution amended with neutral red and sodium alginate can lower the redox potential and reduce hexachloro-1,3-butadiene concentrations in a contaminated vadose zone soil.

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References

  • Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402

    Article  CAS  PubMed  Google Scholar 

  • Cassidy MB, Lee H, Trevors JT (1997) Survival and activity of lac-lux marked Pseudomonas aeruginosa UG2Lr cells encapsulated in κ-carrageenan over four years at 4°C. J Microbiol Methods 30(2):167–170

    Article  CAS  Google Scholar 

  • Coviello T, Matricardi P, Marianecci C, Alhaique F (2007) Polysaccharide hydrogels for modified release formulations. J Colloid Release 119:5–24

    Article  CAS  Google Scholar 

  • Cronk JK, Fennessy MS (2001) Wetland plants: biology and ecology. CRC Press LLC, Boca Raton

    Book  Google Scholar 

  • Engler RM Jr, Patrick WH (1973) Sulfate reduction and sulfide oxidation in flooded soil as affected by chemical oxidants. Soil Sci Soc Am J 37:685–688

    Article  CAS  Google Scholar 

  • Ferguson JF, Pietari JMH (2000) Anaerobic transformations and bioremediation of chlorinated solvents. Environ Pollut 107(2):209–215

    Article  CAS  PubMed  Google Scholar 

  • Gentry TJ, Rensing C, Pepper IL (2004) New approaches for bioaugmentation as a remediation technology. Crit Rev Environ Sci Technol 34(5):447–494

    Article  CAS  Google Scholar 

  • James D, Cord-Ruwisch R, Schleheck D, Lee M, Manefield M (2008) Cyanocobalamin enables activated sludge bacteria to dechlorinate Hexachloro-1, 3-butadiene to nonchlorinated Gases. Bioremediat J 12(4):177–184

    Article  CAS  Google Scholar 

  • Lecloux A (2004) Hexachlorobutadiene—sources, environmental fate and risk characteristics. Sci Doss 5:1–33

    Google Scholar 

  • Manefield M, Whiteley AS, Griffiths RI, Bailey M (2002) RNA stable isotope probing, a novel means of linking microbial community function to phylogeny. Appl Environ Microbiol 68:5367–5373

    Article  CAS  PubMed  Google Scholar 

  • Moslemy P, Guiot S, Neufeld R (2004) Activated sludge encapsulation in gellan gum microbeads for gasoline biodegradation. Bioprocess Biosyst Eng 26(4):1–8

    Article  Google Scholar 

  • Shi Lei, Harms Hauke, Wick LukasY (2008) Electroosmotic flow stimulates the release of alginate-bound phenanthrene. Environ Sci Technol 42(6):2105–2110

    Article  CAS  PubMed  Google Scholar 

  • So CM, Young LY (1999) Isolation and characterisation of a sulfate-reducing bacterium that anaerobically degrades alkanes. Appl Environ Microbiol 65:2969–2976

    CAS  PubMed  Google Scholar 

  • Sriamornsak P, Sungthongjeen S (2007) Modification of theophylline release with alginate gel formed in hard capsules. AAPS PharmSciTech 8(3), Article 51 (E1–E8)

  • Van der Zee FP, Cervantes FJ (2009) Impact and application of electron shuttles on the redox (bio) transformation of contaminants: a review. Biotechnol Adv 27(3):256–277

    Article  PubMed  Google Scholar 

  • Whiteley A, Bailey MJ (2000) Bacterial community structure and physiological state within an industrial phenol bioremediation system. Appl Environ Microbiol 66:2400–2407

    Article  CAS  PubMed  Google Scholar 

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Acknowledgements

This work was supported by the Environmental Biotechnology Cooperative Research Centre and Orica Australia Pty Ltd. Mike Manefield, Lachlan Yee and Matthew Lee were supported by postdoctoral fellowships. Vibeke Aagaard and Angela Johnstone were supported by student stipends.

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Authors and Affiliations

  1. Centre for Marine Bio-Innovation, The University of New South Wales, Sydney, NSW, 2052, Australia

    Lachlan H. Yee, Vibeke Aagaard, Angela Johnstone, Matthew Lee, Staffan J. Kjelleberg & Mike Manefield

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  1. Lachlan H. Yee
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  2. Vibeke Aagaard
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  3. Angela Johnstone
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  4. Matthew Lee
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  5. Staffan J. Kjelleberg
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  6. Mike Manefield
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Correspondence to Mike Manefield.

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Yee, L.H., Aagaard, V., Johnstone, A. et al. Development of a treatment solution for reductive dechlorination of hexachloro-1,3-butadiene in vadose zone soil. Biodegradation 21, 947–956 (2010). https://doi.org/10.1007/s10532-010-9354-z

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  • DOI: https://doi.org/10.1007/s10532-010-9354-z

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