Abstract
The potential of recently isolated bacteria Paenibacillus larvae for the effective decolorization of Indigo carmine was evaluated. The effects of operational parameters (temperature, pH, dye concentration, shaking/non shaking) were tested. Maximum extent of decolorization was observed when the medium was incorporated with 10 g/l of yeast extract and peptone. Decolorization was strongly inhibited at non-shaken conditions as well as incorporation of inorganic sources (sodium nitrite and ammonium chloride) in the medium. Maximum decolorization was observed at 30°C (100%) and 40°C (92%) at 8 h of incubation. The LC-MS and NMR analysis confirms the oxidation of Indigo carmine .The primary degradation products were found to be Isatin sulfonic acid and anthranilicacid.
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Ramya, M., Anusha, B. & Kalavathy, S. Decolorization and biodegradation of Indigo carmine by a textile soil isolate Paenibacillus larvae . Biodegradation 19, 283–291 (2008). https://doi.org/10.1007/s10532-007-9134-6
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DOI: https://doi.org/10.1007/s10532-007-9134-6