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Enhanced production of ligninolytic enzymes and decolorization of molasses distillery wastewater by fungi under solid state fermentation

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Abstract

Selected isolates of fungi were grown on wheat straw and corncob in the presence of different moistening agents such as water, molasses, potato dextrose broth and distillery effluent. All the fungal isolates responded differently with respect to growth and ligninolytic enzyme production. Fungal growth on different substrates was checked by calculating ergosterol content, which varied widely within a single species when grown on different substrates. The maximum laccase production was obtained for Aspergillus flavus TERI DB9 grown on wheat straw with molasses. For manganese peroxidase, highest production was in Aspergillus niger TERI DB20 grown on corncob with effluent. Among the two isolates positive for lignin peroxidase, the highest production was in Fusarium verticillioides ITCC 6140. This immobilized fungal biomass was then used for decolorization of effluent from a cane molasses based distillery. Maximum decolorization (86.33%) was achieved in Pleurotus ostreatus (Florida) Eger EM 1303 immobilized on corncob with molasses in a period of 28 days.

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Acknowledgments

Authors wish to thank Dr R. K. Pachauri, Director-General, TERI and Chancellor, TERI University, New Delhi, India for offering the infrastructure for carrying out the present investigation. Financial assistance from University Grants Commission, New Delhi in the form of Senior Research Fellowship to the first author is duly acknowledged.

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  1. Centre of Bioresources and Biotechnology, TERI University, DS Block, India Habitat Centre, Lodhi Road, New Delhi, 110003, India

    Deepak Pant & Alok Adholeya

  2. Biotechnology and Management of Bioresources Division, TERI, DS Block, India Habitat Centre, Lodhi Road, New Delhi, 110003, India

    Alok Adholeya

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  1. Deepak Pant
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Correspondence to Alok Adholeya.

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Pant, D., Adholeya, A. Enhanced production of ligninolytic enzymes and decolorization of molasses distillery wastewater by fungi under solid state fermentation. Biodegradation 18, 647–659 (2007). https://doi.org/10.1007/s10532-006-9097-z

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