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Isolation and characterization of a novel bacterium, Sphingomonas bisphenolicum strain AO1, that degrades bisphenol A

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Abstract

Bisphenol A (2,2-bis(4-hydroxyphenyl) propane, BPA), which is used as a synthetic resin material or a plasticizer, is a pollutant that␣possesses endocrine-disrupting activity. Bioremediation of BPA is used to decrease its polluting effects, and here we report a novel bacterial strain AO1, which is able to degrade BPA. This strain was isolated using enrichment cultivation from a soil sample from a vegetable-growing field; the sample was one of 500 soil samples collected across Japan. Strain AO1 degraded 100 mg/l BPA to an undetectable level within 6 h in MYPG medium (containing malt extract, yeast extract, peptone, and glucose) and within 48 h in minimum medium containing 1% glucose at 30°C. Strain AO1 can utilize BPA as a sole source of carbon and as an energy source under aerobic conditions. The estrogenic activity of BPA in MYPG medium was ultimately reduced by strain AO1, although the activity initially increased. Taxonomical analysis showed that strain␣AO1 is closely related to Sphingomonas chlorophenolicum and S. herbicidovorans, neither of which have a capacity for BPA degradation. DNA–DNA hybridization showed that strain AO1 is a novel species of the Sphingomonas genus, and we designated AO1 as S. bisphenolicum.

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Acknowledgements

We thank Ms. Akiko Oishi for her technical assistance. The authors thank Dr. Osamu Shida, R&D Department, Higeta Shoyu Co. Ltd., Chiba, Japan for physiological and biochemical characterization of strain AO1.

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Authors and Affiliations

  1. Tsukuba Research Institute, Sumitomo Forestry Co., Ltd., Midorigahara 3-2, Tsukuba, Ibaraki, 300-2646, Japan

    Ko-ichi Oshiman

  2. Department of Forest and Forest Product Sciences, Kyushu University, Hakozaki 6-10-1, Higashi-ku, Fukuoka, 812-8581, Japan

    Yuji Tsutsumi

  3. Department of Forest Resources Science, Shizuoka University, Ohya 836, Suruga-ku, Shizuoka, 422-8529, Japan

    Tomoaki Nishida

  4. Department of Biotechnology, Faculty of␣Engineering, and High Technology Research Center, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka, 564-8680, Japan

    Yoshinobu Matsumura

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  1. Ko-ichi Oshiman
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  2. Yuji Tsutsumi
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  3. Tomoaki Nishida
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  4. Yoshinobu Matsumura
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Correspondence to Ko-ichi Oshiman.

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Oshiman, Ki., Tsutsumi, Y., Nishida, T. et al. Isolation and characterization of a novel bacterium, Sphingomonas bisphenolicum strain AO1, that degrades bisphenol A. Biodegradation 18, 247–255 (2007). https://doi.org/10.1007/s10532-006-9059-5

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  • DOI: https://doi.org/10.1007/s10532-006-9059-5

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