Abstract
Objective
The present investigation primarily focusses on enhancement of resveratrol production by endophytic production from the endophytic fungus, Arcopilus aureus via one variable at a time approach (OVAT) followed by statistical approach using response surface methodology (RSM). The paper also highlights the characterization of fungal resveratrol using spectroscopic techniques. Further the tyrosinase inhibitory property was also explored in this communication for its possible use as a cosmeceutical ingredient.
Results
Optimization of physicochemical and nutritional parameters using OVAT approach exhibited 1.23-fold enhancement in production of resveratrol when compared to initial yield, 89.1 ± 0.08 µg/mL. Further RSM resulted in 1.49-fold enhancement in resveratrol production i.e. 133.53 µg/ml. Further, 25 mg of fungal resveratrol in pure form was obtained from the spent broth of Arcopilus aureus by column chromatography using a mobile phase comprising of MeOH: DCM in a ratio of 1.75:98.25. Further its purity on TLC was checked using 5% MeOH: DCM as mobile phase. Symmetrical peak with Rt of 3.36 min using a C18 reverse phase column confirmed the homogeneity of the purified fungal resveratrol with standard resveratrol and further corroborated by 1H-NMR, 13C-NMR and HR-MS analysis. Fungal resveratrol exhibited a good tyrosinase inhibition with an IC50 of 2.654 ± 0.432 µg/mL as compared to Kojic acid (1.329 ± 0.333).
Conclusions
The present study has provided sufficient lead that process optimization techniques can complement each other for optimized production of bioactive compounds by microorganisms apart from strain improvement techniques which are generally adopted in the industry. The enhancement of resveratrol production by Arcopilus aureus by process optimization further opens up avenues for strain improvement for commercial resveratrol production through fermentation for nutraceutical and cosmeceutical applications.
Similar content being viewed by others
References
Dwibedi V, Saxena S (2018) Arcopilus aureus, a resveratrol-producing endophyte from Vitis vinifera. Appl Biochem Biotechnol 186(2):476–495
Fan E, Zhang K, Zhu M, Wang Q (2010) Obtaining resveratrol: from chemical synthesis to biotechnological production. Mini-Rev Org Chem 7(4):272–281
Garyali S, Kumar A, Reddy MS (2014) Enhancement of taxol production from endophytic fungus Fusarium redolens. Biotechnol Bioproc E 19(5):908–915
Goutam J, Sharma VK, Verma SK, Singh DK, Kumar J, Mishra A, Kumar A, Kharwar R (2014) Optimization of culture conditions for enhanced production of bioactive metabolites rich in antimicrobial and antioxidant activities isolated from Emericella quadrilineata an endophyte of Pteris pellucida. J Pure Appl Microbiol 8(3):2059–2073
Hubbard BP, Sinclair DA (2014) Small molecule SIRT1 activators for the treatment of aging and age-related diseases. Trends Pharmacol Sci 35(3):146–154
Jeandet P, Delaunois B, Aziz A, Donnez D, Vasserot Y, Cordelier S, Courot E (2012) Metabolic engineering of yeast and plants for the production of the biologically active hydroxystilbene, resveratrol. J Biomed Res 2012:579089
Kou X, Chen N (2017) Resveratrol as a natural autophagy regulator for prevention and treatment of Alzheimer’s disease. Nutrients 9(9):927
Liu Y, Zhou J, Qu Y, Yang X, Shi G, Wang X, Hong Y, Drlica K, Zhao X (2016) Resveratrol antagonizes antimicrobial lethality and stimulates recovery of bacterial mutants. PLoS ONE 11(4):e0153023
Myers RH, Montgomery DC (2002) Response surface methodology: product and process optimization using designed experiments, 2nd edn. Wiley, New York
Parage C, Tavares R, Réty S, Baltenweck-Guyot R, Poutaraud A, Renault L, Heintz D, Lugan R, Marais GA, Aubourg S (2012) Structural, functional, and evolutionary analysis of the unusually large stilbene synthase gene family in grapevine. Plant Physiol 160(3):1407–1419
Qiao G, Guo B, Ou J (2017) Numerical simulation to optimize impressed current cathodic protection systems for rc structures. J Mater Civil Eng 29(6):04017005
Rajput H, Dhir A, Kumar Sangal V (2018) GO mediated TiO2 nanotube electrode for the photoelectrocatalytic degradation of pentachlorophenol. J Electrochem Soc 165(2):H16–H26
Saran S, Mukherjee S, Dalal J, Saxena RK (2015) High production of erythritol from Candida sorbosivorans SSE-24 and its inhibitory effect on biofilm formation of Streptococcus mutans. Bioresour Technol 198:31–38
Saxena S, Srivastava A (2014) Resveratrol: biological activities and therapeutic potential. J Pharm Technol Res Manage 2(2):145–157
Shi J, Zeng Q, Liu Y, Pan Z (2012) Alternaria sp. MG1, a resveratrol-producing fungus: isolation, identification, and optimal cultivation conditions for resveratrol production. Appl Microbiol Biotechnol. 95(2):369–379
Tassoni A, Fornalè S, Franceschetti M, Musiani F, Michael AJ, Perry B, Bagni N (2005) Jasmonates and Na-orthovanadate promote resveratrol production in Vitis vinifera cv Barbera cell cultures. New Phytol 166(3):895–905
Turrini E, Calcabrini C, Tacchini M, Efferth T, Sacchetti G, Guerrini A, Paganetto G, Catanzaro E, Greco G, Fimognari C (2018) In vitro study of the cytotoxic, cytostatic, and antigenotoxic profile of Hemidesmus indicus (L.) R. Br(Apocynaceae) crude drug extract on T lymphoblastic cells. Toxins 10(2):70
Vannozzi A, Dry IB, Fasoli M, Zenoni S, Lucchin M (2012) Genome-wide analysis of the grapevine stilbene synthase multigenic family: genomic organization and expression profiles upon biotic and abiotic stresses. BMC Plant Biol 12(1):130
Wang D-G, Liu W-Y, Chen G-T (2013) A simple method for the isolation and purification of resveratrol from Polygonum cuspidatum. J Pharm Anal 3(4):241–247
Yun TY, Feng RJ, Zhou DB, Pan YY, Chen YF, Wang F, Yin LY, Zhang YD, Xie JH (2018) Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1–14 from cassava rhizosphere. PLoS ONE 13(11):e0206497
Zhou Z, Wang M, Guo Z, Zhang X (2016) Pharmacokinetic evaluation of the interaction between oral kaempferol and ethanol in rats. Acta Pharmaceut 66(4):563–568
Zhou D, Xu P, Gu Q (2018) Finding local minima via stochastic nested variance reduction. https://arxiv.org/abs/180608782
Zhu T, Heo HJ, Row KH (2010) Optimization of crude polysaccharides extraction from Hizikia fusiformis using response surface methodology. Carbohyd Polym 82(1):106–110
Acknowledgements
The Authors thank Department of Biotechnology and TIFAC-CORE (Centre of Relevance and Excellence), Thapar Institute of Engineering and Technology, Patiala, Punjab for providing the necessary infrastructure to carry out the research work.
Funding
No funding.
Author information
Authors and Affiliations
Contributions
VD and SS both conceived and designed the experiments; VD, performed all the experiments. SKR and RP collaborated for analytical chemistry and optimized the purification and characterization. VD and SS analysed the data and wrote the paper.
Corresponding authors
Ethics declarations
Conflict of interest
Authors declares that they have no conflict of interest.
Research involving human and animal rights
This article does not contain any studies with human participants or animals.
Informed consent
Informed consent is not required for this study.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Dwibedi, V., Rath, S.K., Prakash, R. et al. Response surface statistical optimization of fermentation parameters for resveratrol production by the endophytic fungus Arcopilus aureus and its tyrosinase inhibitory activity. Biotechnol Lett 43, 627–644 (2021). https://doi.org/10.1007/s10529-020-03032-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-020-03032-7