Abstract
Objective
To develop an efficient synthetic promoter library for fine-tuned expression of target genes in Corynebacterium glutamicum.
Results
A synthetic promoter library for C. glutamicum was developed based on conserved sequences of the − 10 and − 35 regions. The synthetic promoter library covered a wide range of strengths, ranging from 1 to 193% of the tac promoter. 68 promoters were selected and sequenced for correlation analysis between promoter sequence and strength with a statistical model. A new promoter library was further reconstructed with improved promoter strength and coverage based on the results of correlation analysis. Tandem promoter P70 was finally constructed with increased strength by 121% over the tac promoter. The promoter library developed in this study showed a great potential for applications in metabolic engineering and synthetic biology for the optimization of metabolic networks.
Conclusions
To the best of our knowledge, this is the first reconstruction of synthetic promoter library based on statistical analysis of C. glutamicum.
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Acknowledgements
This work was supported by National Natural Science Foundation of China (NSFC-21576200, NSFC-21776209, NSFC-21621004 and NSFC-21390201) and Natural Science Foundation of Tianjin (No. 15JCQNJC06000).
Supporting information
Supplementary Table 1—Sequence of the individual promoters.
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Shuanghong Zhang and Dingyu Liu are the first authors.
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Zhang, S., Liu, D., Mao, Z. et al. Model-based reconstruction of synthetic promoter library in Corynebacterium glutamicum. Biotechnol Lett 40, 819–827 (2018). https://doi.org/10.1007/s10529-018-2539-y
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DOI: https://doi.org/10.1007/s10529-018-2539-y