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Copper oxide nanoparticles induce anticancer activity in A549 lung cancer cells by inhibition of histone deacetylase

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Abstract

Objectives

Copper oxide nanoparticles (CuO NPs) promoting anticancer activity may be due to the regulation of various classes of histone deacetylases (HDACs).

Results

Green-synthesized CuO NPs significantly arrested total HDAC level and also suppressed class I, II and IV HDACs mRNA expression in A549 cells. A549 cells treated with CuO NPs downregulated oncogenes and upregulated tumor suppressor protein expression. CuO NPs positively regulated both mitochondrial and death receptor-mediated apoptosis caspase cascade pathway in A549 cells.

Conclusion

Green-synthesized CuO NPs inhibited HDAC and therefore shown apoptosis mediated anticancer activity in A549 lung cancer cell line.

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Acknowledgements

We thank the DST-FIST for their infrastructure support to our department. The first author thanks to Dr. K. Jayaraman, Department of Educational Technology, Bharathidasan University, Tiruchirappalli, for the constant encouragement and financial support throughout her career. The authors are grateful to Dr. C. Prahalathan and Dr. A. Antony Joseph Velanganni, Department of Biochemistry, Bharathidasan University, Tiruchirappalli, India for his help with gel documentation and fluorescence studies. Sincere thanks to Dr. S. Sivaramakrishnan, Department of Biotechnology and Genetic Engineering, for his help with gel documentation studies and cell storage.

Supporting information

Supplementary Fig. 1—Field emission-scanning electron microscopic analysis of a CuO NPs b EDAX spectrum (CuO NPs- Copper oxide nanoparticles; EDAX- Energy dispersive X-ray spectroscopy).

Supplementary Table 1—The sequence of the primers used for the RT-PCR (RT-PCR- Reverse transcription PCR).

Author information

Author notes

  1. Arunachalam Kalaiarasi and Renu Sankar have contributed equally to this study.

Authors and Affiliations

  1. Department of Biochemistry, School of Life Sciences, Bharathidasan University, Tiruchirappalli, 620 024, Tamil Nadu, India

    Arunachalam Kalaiarasi, Renu Sankar, Chidambaram Anusha, Kandasamy Saravanan, Kalyanasundaram Aarthy & Vilwanathan Ravikumar

  2. Food Animal Health Research Program, Ohio Agricultural Research and Development Center, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA

    Renu Sankar

  3. Division of Drug Research/Clinical Pharmacology, Department of Medical and Health Sciences, Linköping University Hospital, 581 83, Linköping, Sweden

    Selvaraj Karthic

  4. Department of Cellular and Molecular Biochemistry, Frontier Mediville (A Unit of Frontier Lifeline and Dr. K. M. Cherian Heart Foundation), Affiliated to University of Madras, Chennai, 601201, Tamil Nadu, India

    Theodore lemuel Mathuram

Authors
  1. Arunachalam Kalaiarasi
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  2. Renu Sankar
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  3. Chidambaram Anusha
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  4. Kandasamy Saravanan
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  5. Kalyanasundaram Aarthy
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  7. Theodore lemuel Mathuram
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  8. Vilwanathan Ravikumar
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Corresponding author

Correspondence to Vilwanathan Ravikumar.

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The authors declare that they have no conflicts of interest concerning this article.

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Kalaiarasi, A., Sankar, R., Anusha, C. et al. Copper oxide nanoparticles induce anticancer activity in A549 lung cancer cells by inhibition of histone deacetylase. Biotechnol Lett 40, 249–256 (2018). https://doi.org/10.1007/s10529-017-2463-6

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  • DOI: https://doi.org/10.1007/s10529-017-2463-6

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