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Inactivation of virginiamycin by Aureobasidium pullulans

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Abstract

Objective

To test the inactivation of the antibiotic, virginiamycin, by laccase-induced culture supernatants of Aureobasidium pullulans.

Results

Fourteen strains of A. pullulans from phylogenetic clade 7 were tested for laccase production. Three laccase-producing strains from this group and three previously identified strains from clade 5 were compared for inactivation of virginiamycin. Laccase-induced culture supernatants from clade 7 strains were more effective at inactivation of virginiamycin, particularly at 50 °C. Clade 7 strain NRRL Y-2567 inactivated 6 µg virginiamycin/ml within 24 h. HPLC analyses indicated that virginiamycin was degraded by A. pullulans.

Conclusions

A. pullulans has the potential for the bioremediation of virginiamycin-contaminated materials, such as distiller’s dry grains with solubles (DDGS) animal feed produced from corn-based fuel ethanol production.

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Authors and Affiliations

  1. Renewable Product Technology Research Unit, Agricultural Research Service, US Department of Agriculture, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL, 61604, USA

    Timothy D. Leathers, Joseph O. Rich, Melinda S. Nunnally & Amber M. Anderson

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  1. Timothy D. Leathers
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  2. Joseph O. Rich
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  3. Melinda S. Nunnally
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  4. Amber M. Anderson
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Corresponding author

Correspondence to Timothy D. Leathers.

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Mention of any trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the US Department of Agriculture. USDA is an equal opportunity provider and employer.

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Leathers, T.D., Rich, J.O., Nunnally, M.S. et al. Inactivation of virginiamycin by Aureobasidium pullulans . Biotechnol Lett 40, 157–163 (2018). https://doi.org/10.1007/s10529-017-2454-7

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  • DOI: https://doi.org/10.1007/s10529-017-2454-7

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