Abstract
Objectives
To investigate the roles of miR-149 in the progression of human osteosarcoma (OS).
Results
miR-149 level was upregulated in tissues from OS patients more than in normal subjects. Cell proliferation and apoptosis assays revealed that miR-149 increased cell proliferation and inhibited cell apoptosis in OS cell line (MG63). An increase of Bcl-2 gene expression and a decrease of cleaved-caspase-3, and cleaved-PARP expression were observed in MG63 cells with transfection of miR-149. Additionally, bone morphogenetic protein 9 (BMP9) was identified as a target of miR-149 in MG63 cells, and BMP9 expression was negatively correlated with miR149 level in OS clinical samples. Co-overexpression of BMP9 with miR-149 in MG63 cells prohibited miR-149-mediated promotive effects on OS progression. Importantly, overexpression of miR-149 conferred chemoresistance in MG63 cells.
Conclusions
miR-149 promotes OS progression via targeting BMP9.
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Supporting information
Supplementary Table 1—shRNA for BMP9 and PCR Primer sequences.
Supplementary Figure 1—The pictures of Amplification Plot and Melt Curve of BMP9, GAPDH and miR-149.
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Xie, Z., Xu, J., Peng, L. et al. miR-149 promotes human osteocarcinoma progression via targeting bone morphogenetic protein 9 (BMP9). Biotechnol Lett 40, 47–55 (2018). https://doi.org/10.1007/s10529-017-2445-8
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DOI: https://doi.org/10.1007/s10529-017-2445-8