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Gelatin microspheres containing calcitonin gene-related peptide or substance P repair bone defects in osteoporotic rabbits

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Abstract

Objectives

To investigate the therapeutic effect of gelatin microspheres containing different concentrations of calcitonin gene-related peptide (CGRP) or substance P on repairing bone defects in a rabbit osteoporosis model.

Results

Gelatin microspheres containing different concentrations of CGRP or substance P promoted osteogenesis after 3 months in a rabbit osteoporotic bone defective model. From micro-computed tomography imaging results, 10 nM CGRP was optimal for increasing the trabecular number and decreasing the trabecular bone separation degree; similar effects were observed with the microspheres containing 1 µM substance P. Histological analysis showed that the gelatin microspheres containing CGRP or substance P, regardless of the concentration, effectively promoted osteogenesis, and the highest effect was achieved in the groups containing 1 µM CGRP or 1 µM substance P.

Conclusions

Gelatin microspheres containing CGRP or substance P effectively promoted osteogenesis in a rabbit osteoporotic bone defect model dose-dependently, though their effects in repairing human alveolar ridge defects still need further investigation.

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Acknowledgements

This project was supported by funding from the National Natural Science Foundation of China (81271103).

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Authors and Affiliations

  1. State Key Laboratory of Military Stomatology & National Clinical Research Center for Oral Diseases & Shaanxi Clinical Research Center of Oral Diseases, Department of Oral Surgery, School of Stomatology, The Fourth Military Medical University, 145 Western Changle Road, Xi’an, 710032, People’s Republic of China

    Jianghao Chen, Wei Liu, Jie Chen, Kaijin Hu, Linlin Zhang & Yuxiang Ding

  2. Department of Stomatology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, People’s Republic of China

    Jinxiu Zhao & Cong Sun

Authors
  1. Jianghao Chen
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  2. Wei Liu
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  3. Jinxiu Zhao
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  4. Cong Sun
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  5. Jie Chen
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  6. Kaijin Hu
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  7. Linlin Zhang
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  8. Yuxiang Ding
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Corresponding author

Correspondence to Yuxiang Ding.

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All authors declare that there are no conflicts of interest.

Ethical approval

Procedures were carried out according to the Guidelines for the Care and Use of Laboratory Animals and complied with regulations of the Animal Research Committee of our institution.

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Chen, J., Liu, W., Zhao, J. et al. Gelatin microspheres containing calcitonin gene-related peptide or substance P repair bone defects in osteoporotic rabbits. Biotechnol Lett 39, 465–472 (2017). https://doi.org/10.1007/s10529-016-2263-4

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  • DOI: https://doi.org/10.1007/s10529-016-2263-4

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