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Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells

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Abstract

Objective

To design and synthesize a novel near-infrared (NIR) fluorescent probe based on indocyanine Green (ICG), that can be applied in imaging living cells.

Results

A highly fluorescent novel NIR fluorescent probe (IR-793) was synthesized in two steps. IR-793 had better fluorescence and optical stability than ICG. In addition, no obvious cytotoxicity effect of IR-793 was observed and cell viability was above 75% at the maximum concentration (120 nM). IR-793 also exhibited good performance in imaging living A549 cells.

Conclusion

IR-793, a novel NIR fluorescent probe that is stable, low-cost, highly fluorescent and low cytotoxicity, has been designed and synthesized for imaging living cells.

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Acknowledgments

This work is supported by National High-tech Research and Development Project (863 Project, 2013AA032205), Industry Project of Jiangsu Science-technology Support Plan (BE2013840), Science and Technology Development Program of Suzhou (ZXY201412), the Graduate Research and Innovation Program of Jiangsu Province in China (KYLX15-0170).

Supporting information

Supplementary Figure—1H-NMR and MS of IR-793.

Author information

Authors and Affiliations

  1. School of Biological Science & Medical Engineering, Southeast University, Nanjing, 210096, China

    Jing Xing, Gaoxin Zhou, Bo Chen & Min Ji

  2. School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou, 215123, China

    Jing Xing, Gaoxin Zhou, Bo Chen & Min Ji

  3. College of Life Sciences, Binzhou University, Binzhou, 256600, China

    Chunlong Sun

  4. Chia-tai Tianqing Pharmaceutical Group Co. Ltd., No.699-8 Xuanwu Blvd., Nanjing, 210042, China

    Huanqing Zhang

  5. School of Chemistry & Chemical Engineering, Southeast University, Nanjing, 210096, China

    Xi Zong & Jin Cai

Authors
  1. Jing Xing
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  2. Gaoxin Zhou
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  3. Chunlong Sun
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  4. Huanqing Zhang
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  5. Bo Chen
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  6. Xi Zong
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  7. Jin Cai
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  8. Min Ji
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Corresponding author

Correspondence to Min Ji.

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Supplementary material 1 (DOCX 194 kb)

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Cite this article

Xing, J., Zhou, G., Sun, C. et al. Synthesis and characterization of a novel near-infrared fluorescent probe for applications in imaging A549 cells. Biotechnol Lett 38, 1851–1856 (2016). https://doi.org/10.1007/s10529-016-2179-z

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  • DOI: https://doi.org/10.1007/s10529-016-2179-z

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