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Enterococcus faecalis promotes osteoclast differentiation within an osteoblast/osteoclast co-culture system

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Abstract

Objective

To investigate the effects of heat-killed Enterococcus faecalis ATCC 29212 and P25RC clinical strain (derived from an obturated root canal with apical periodontitis) on osteoclast differentiation within an osteoblast/osteoclast co-culture system.

Results

Heat-killed E. faecalis significantly increased the proportion of multinucleated osteoclastic cells (MNCs) within the co-culture system. The IL-6 level was significantly increased upon exposure to heat-killed E. faecalis. Gene expression levels of NFATc1 and cathepsin K were significantly up-regulated compared to the untreated control. EphrinB2 and EphB4 expressions at both the mRNA and protein levels were also significantly upregulated compared to the untreated control.

Conclusions

Heat-killed E. faecalis can induce osteoclast differentiation within the osteoblast/osteoclast co-culture system in vitro, possibly through ephrinB2-EphB4 bidirectional signaling.

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Acknowledgments

This work was supported by RFCID/HHSRF HMRF Grant No. 12110772 and Shenzhen Key Laboratory of ENT (No. ZDSYS 201506050935272).

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    Authors and Affiliations

    1. Endodontics, Comprehensive Dental Care, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, 999077, China

      Zuhui Deng, Shuai Wang, Boon Chin Heng, Changyong Yuan & Chengfei Zhang

    2. Shenzhen ENT Institute, Shenzhen Longgang ENT Hospital, Shenzhen, 518172, Guangdong, China

      Zuhui Deng

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    1. Zuhui Deng
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    2. Shuai Wang
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    3. Boon Chin Heng
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    Correspondence to Chengfei Zhang.

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    The authors declare that there are no conflicts of interest.

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    Zuhui Deng and Shuai Wang have contributed equally to this work.

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    Deng, Z., Wang, S., Heng, B.C. et al. Enterococcus faecalis promotes osteoclast differentiation within an osteoblast/osteoclast co-culture system. Biotechnol Lett 38, 1443–1448 (2016). https://doi.org/10.1007/s10529-016-2142-z

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    • DOI: https://doi.org/10.1007/s10529-016-2142-z

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