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Gene expression pattern analysis of a recombinant Escherichia coli strain possessing high growth and lycopene production capability when using fructose as carbon source

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Abstract

Objective

Escherichia coli K12f-pACLYC has a high capability for growth and lycopene production when using fructose as carbon source and the transcription of genes involved was compared in glucose-grown and fructose-grown cells.

Results

Escherichia coli K12f-pACLYC was grown on 10 g fructose l−1 and reached 4.6 g DCW l−1 with lycopene at 192 mg g DCW−1, values that are 3-fold and 7-fold higher than when growing on glucose. Gene transcription profiles of fructose-grown and glucose-grown cells were compared. 384 differentially expressed genes (DEGs) with fold changes ≥4 were identified, and the transcription of genes involved in fructose uptake and metabolism, pyruvate catabolism, tricarboxylic acid cycle and oxidative phosphorylation varied significantly. These changes enhanced the metabolic flux into the Embden–Meyerhof–Parnas pathway and the tricarboxylic acid cylcle and coupled to oxidative phosphorylation. These enhanced activities provide more precursors, cofactors and energy needed for growth lycopene production.

Conclusion

The high capability of E. coli K12f-pACLYC for growth and lycopene production when growing on fructose is due to transcriptional regulation, and the relevant genes were identified.

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Acknowledgments

This work was supported by the foundation of the Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education and the Tianjin Key Lab of Industrial Microbiology (Tianjin University of Science and Technology) (No. 2013IM102).

Supplementary information

Supplementary Table 1—Primers used in quantitative real-time (qRT) PCR analysis.

Supplementary Table 2—Gene ID, name, annotation, and expression pattern and of all the DEGs mentioned involved in the paper.

Supplemetary Fig. 1—COG function classifications of DEGs.

Supplemetary Fig. 2—KEGG function classifications of DEGs.

Author information

Authors and Affiliations

  1. College of Biotechnology, Tianjin University of Science and Technology, No. 29, 13th Street, Tianjin Economic and Technological Development Area, Tianjin, 300457, China

    Wen Du, Yajian Song, Mengya Liu, Haixu Yang, Yao Zhang, Yanli Fan, Xuegang Luo, Zhongyuan Li, Nan Wang, Hongpeng He, Hao Zhou, Wenjian Ma & Tongcun Zhang

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  1. Wen Du
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  2. Yajian Song
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  3. Mengya Liu
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  4. Haixu Yang
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  5. Yao Zhang
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  6. Yanli Fan
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  7. Xuegang Luo
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  8. Zhongyuan Li
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  9. Nan Wang
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  10. Hongpeng He
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  11. Hao Zhou
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  12. Wenjian Ma
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  13. Tongcun Zhang
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Corresponding author

Correspondence to Tongcun Zhang.

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Du, W., Song, Y., Liu, M. et al. Gene expression pattern analysis of a recombinant Escherichia coli strain possessing high growth and lycopene production capability when using fructose as carbon source. Biotechnol Lett 38, 1571–1577 (2016). https://doi.org/10.1007/s10529-016-2133-0

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  • DOI: https://doi.org/10.1007/s10529-016-2133-0

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