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COMP-Ang1 promotes long-term survival of allogeneic islet grafts in a bioinert perforated chamber by inhibiting inflammation via inhibition of the TLR4 signaling pathway

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Abstract

Objectives

To evaluate the effects of cartilage oligomeric matrix protein (COMP)- angiopoietin-1 (Ang1) on allogeneic islet graft survival in a bioinert perforated chamber.

Results

COMP-Ang1 treatment significantly decreased lipopolysaccharide-induced cell apoptosis and islet-related lymph node cell proliferation (both P < 0.01). Tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 levels in the chamber exudate were significantly lower in the COMP-Ang1 + chamber group than in the chamber group (all P < 0.05), as were the protein expression levels. COMP-Ang1 significantly inhibited the expression of Toll-like receptor 4 (TLR4) in cultured islets. Finally, full COMP-Ang1 treatment resulted in the longest survival time among the treatment groups.

Conclusion

Combined use of the bioinert perforated chamber with COMP-Ang1 is an effective strategy for improving islet allograft survival.

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Funding

This work was supported by the Foundation of Xijing Hospital (XJZT13Z03) and the National Natural Science Foundation of China (81500450, L.N.J.).

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Author notes

    Authors and Affiliations

    1. Institute of Plastic Surgery, Fourth Military Medical University, Xijing Hospital, Xi’an, 710032, People’s Republic of China

      Dalei Wang, Yingzi Liang, Xiaoyan Hao, Chen Chen, Wei Xia, Yingjun Su & Shuzhong Guo

    2. Department of Pathology and Hepatology, Beijing 302 Hospital, Beijing, 100039, People’s Republic of China

      Lina Jiang

    3. Department of Immunology, The Fourth Military Medical University, Xi’an, 710032, People’s Republic of China

      Ran Zhuang

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    1. Dalei Wang
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    2. Lina Jiang
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    Correspondence to Shuzhong Guo.

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    The authors declare that they have no conflicts of interest concerning this article.

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    Dalei Wang and Lina Jiang have contributed equally to this work.

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    Wang, D., Jiang, L., Liang, Y. et al. COMP-Ang1 promotes long-term survival of allogeneic islet grafts in a bioinert perforated chamber by inhibiting inflammation via inhibition of the TLR4 signaling pathway. Biotechnol Lett 38, 1033–1042 (2016). https://doi.org/10.1007/s10529-016-2059-6

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    • DOI: https://doi.org/10.1007/s10529-016-2059-6

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