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Cell line development for biomanufacturing processes: recent advances and an outlook

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Abstract

At the core of a biomanufacturing process for recombinant proteins is the production cell line. It influences the productivity and product quality. Its characteristics also dictate process development, as the process is optimized to complement the producing cell to achieve the target productivity and quality. Advances in the past decade, from vector design to cell line screening, have greatly expanded our capability to attain producing cell lines with certain desired traits. Increasing availability of genomic and transcriptomic resources for industrially important cell lines coupled with advances in genome editing technology have opened new avenues for cell line development. These developments are poised to help biosimilar manufacturing, which requires targeting pre-defined product quality attributes, e.g., glycoform, to match the innovator’s range. This review summarizes recent advances and discusses future possibilities in this area.

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    Authors and Affiliations

    1. Amgen Inc., Thousand Oaks, USA

      Huong Le & Nitya M. Jacob

    2. Takeda Pharmaceutical Company Ltd., Osaka, Japan

      Nandita Vishwanathan

    3. Chemical Engineering and Process Development, CSIR-National Chemical Laboratory, Pune, 411008, Maharashtra, India

      Mugdha Gadgil

    4. Department of Chemical Engineering and Materials Science, University of Minnesota, Minneapolis, MN, 55455, USA

      Wei-Shou Hu

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    Correspondence to Wei-Shou Hu.

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    Huong Le and Nandita Vishwanathan have contributed equally to this work.

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    Le, H., Vishwanathan, N., Jacob, N.M. et al. Cell line development for biomanufacturing processes: recent advances and an outlook. Biotechnol Lett 37, 1553–1564 (2015). https://doi.org/10.1007/s10529-015-1843-z

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