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Production of 5,8-dihydroxy-9(Z)-octadecenoic acid from oleic acid by whole recombinant cells of Aspergillus nidulans expressing diol synthase

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Abstract

The optimal conditions for the production of 5,8-dihydroxy-9(Z)-octadecenoic acid (5,8-diHOME) from oleic acid by whole recombinant Escherichia coli cells expressing diol synthase from Aspergillus nidulans were 40 °C, pH 7.5, 10 % (v/v) dimethyl sulfoxide, 35 g cells l−1, and 12 g oleic acid l−1 at 250 rpm in a 250 ml baffled flask. Under these conditions, whole recombinant cells produced 5.2 g 5,8-diHOME l−1 together with 1 g l−1 of the intermediate 8-hydroperoxy-9(Z)-octadecenoic acid (8-HPOME) after 60 min. This corresponded to a conversion yield of 43 % (w/w), a volumetric productivity of 5.2 g l−1 h−1, and a specific productivity of 148 mg g cells−1 h−1. This is the first report of the biotechnological production of 5,8-diHOME from oleic acid.

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Acknowledgments

This study was supported by Grants from the Bio-industry Technology Development Program, Ministry for Agriculture, Food and Rural Affairs (No. 112002-3) and the Korea Healthcare Technology R&D Project, Ministry for Health & Welfare, Republic of Korea (No. 2012-009).

Supporting information

Supplementary Fig. 1—LC–MS/MS spectrum of 8-HPOME obtained from conversion of α-linolenic acid using diol synthase from A. nidulans double-site (H1004A-C1006S) variant

Supplementary Fig. 2—Effect of temperature on the production of 5,8-diHOME from oleic acid using whole recombinant cells expressing diol synthase from A. nidulans

Supplementary Fig. 3—Effect of pH on the production of 5,8-diHOME from oleic acid using whole recombinant cells expressing diol synthase from A. nidulans

Supplementary Fig. 4—Effect of enzyme stability on the production of 5,8-diHOME from oleic acid using whole recombinant cells expressing diol synthase from A. nidulans

Supplementary Fig. 5—Effect of solvents on the production of 5,8-diHOME from oleic acid using whole recombinant cells expressing diol synthase from A. nidulans

Supplementary Fig. 6—Effect of dimethyl sulfoxide concentration on the production of 5,8-diHOME from oleic acid using whole recombinant cells expressing diol synthase from A. nidulans

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Correspondence to Deok-Kun Oh.

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Seo, MJ., Shin, KC., Jeong, YJ. et al. Production of 5,8-dihydroxy-9(Z)-octadecenoic acid from oleic acid by whole recombinant cells of Aspergillus nidulans expressing diol synthase. Biotechnol Lett 37, 131–137 (2015). https://doi.org/10.1007/s10529-014-1650-y

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  • DOI: https://doi.org/10.1007/s10529-014-1650-y

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