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scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry

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Abstract

Infectious bursal disease is an economically important disease that affects chickens worldwide. Here, a recombinant single chain variable fragment (scFv) antibody library derived from chickens immunized with VP2 protein of infectious bursal disease virus (IBDV) was constructed. The library was subjected to three rounds of screening by flow cytometry against VP2 protein through a bacteria display technology, resulting in the enrichment of scFv. Three scFv clones with different fluorescence intensity were obtained by random colony pick up. The isolated scFv antibodies were expressed and purified. Relative affinity assay showed the three clones had different sensitivity to VP2, in accordance with fluorescence activity cell sorting analysis. The potential use of the selected IBDV-specific scFv antibodies was demonstrated by the successful application of the isolated antibodies in western blotting assay and ELISA.

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Acknowledgments

This study was supported by grants of National Science Talents fund subprojects (Z1210069_31) and National Natural Science Foundation of China (31200121/C010802).

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Authors and Affiliations

  1. Heilongjiang River Fishery Research Institute, Chinese Academy of Fishery Sciences, Harbin, 150070, People’s Republic of China

    Li-Ming Xu

  2. College of Life Sciences, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Tian-He Li, Bing Zhou, Mo Guo, Miao Liu, Jing-Zhuang Zhao & De-Shan Li

  3. College of Biological Science and Technology, HeiLongJiang BaYi Agricultural University, DaQing, 163319, China

    Hong-Wei Cao

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  1. Li-Ming Xu
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  2. Tian-He Li
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  3. Bing Zhou
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  4. Mo Guo
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  5. Miao Liu
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  6. Jing-Zhuang Zhao
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  7. Hong-Wei Cao
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  8. De-Shan Li
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Corresponding author

Correspondence to De-Shan Li.

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Li-Ming Xu and Tian-He Li contributed equally to this study.

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Xu, LM., Li, TH., Zhou, B. et al. scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry. Biotechnol Lett 36, 1029–1035 (2014). https://doi.org/10.1007/s10529-014-1463-z

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  • DOI: https://doi.org/10.1007/s10529-014-1463-z

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