Abstract
Over the years, several vectors and host strains have been constructed to improve the overexpression of recombinant proteins in Escherichia coli. More recently, attention has focused on the co-expression of genes in E. coli, either by means of a single vector or by cotransformation with multiple compatible plasmids. Co-expression was initially designed to generate protein complexes in vivo, and later served to extend the use of E. coli as a platform for the production of heterologous proteins. This review shows how the co-expression of genes in E. coli is challenging the production of protein complexes and proteins bearing post-translational modifications or unnatural amino acids. In addition, the importance of co-expression to achieve efficient secretion of recombinant proteins in E. coli is discussed, with recent insights into the use of co-expression to overproduce membrane proteins.
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Hochkoeppler, A. Expanding the landscape of recombinant protein production in Escherichia coli . Biotechnol Lett 35, 1971–1981 (2013). https://doi.org/10.1007/s10529-013-1396-y
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DOI: https://doi.org/10.1007/s10529-013-1396-y