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An ABC transporter complex containing S-adenosylmethionine (SAM)-induced ATP-binding protein is involved in antibiotics production and SAM signaling in Streptomyces coelicolor M145

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An Erratum to this article was published on 21 October 2012

Abstract

A sco3956-deletion mutant (ΔSCO3956) of Streptomyces coelicolor was generated to characterize the S-adenosylmethionine (SAM)-induced, ATP-binding cassette transporter (ABC transporter) ATP-binding protein, SCO3956. It produced actinorhodin (ACT) and undecylprodigiosin (RED) decreased by approx. 82 and 64 %, respectively. In addition, the effect of exogenous SAM was lost in the ΔSCO3956. Plasmid-based complementation of sco3956 in ΔSCO3956 restored ACT and RED levels of ΔSCO3956 to wild-type levels (ACT: 20 ± 1.4 mg g−1 DCW and RED: 5.3 ± 0.6 mg g−1 DCW) and the exogenous effect significantly increased ACT and RED by approx. 129 and 135 %, respectively, when compared to the exogenous SAM non-treated sco3956 complementation strain. Thus, the ABC transporter ATP-binding protein, SCO3956, plays a critical role in ACT and RED production serving as a transducer of SAM signaling.

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Acknowledgments

This work was supported by a grant from the Next-Generation BioGreen 21 Program (No. PJ008093), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. Division of Bioscience and Bioinformatics, Myongji University, Yongin, 449-728, Korea

    Sung-Kwon Lee, SangJoon Mo & Joo-Won Suh

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  1. Sung-Kwon Lee
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  2. SangJoon Mo
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  3. Joo-Won Suh
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Correspondence to Joo-Won Suh.

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Sung-Kwon Lee and SangJoon Mo contributed equally to this study.

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Lee, SK., Mo, S. & Suh, JW. An ABC transporter complex containing S-adenosylmethionine (SAM)-induced ATP-binding protein is involved in antibiotics production and SAM signaling in Streptomyces coelicolor M145. Biotechnol Lett 34, 1907–1914 (2012). https://doi.org/10.1007/s10529-012-0987-3

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  • DOI: https://doi.org/10.1007/s10529-012-0987-3

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