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Endo-xylanase GH11 activation by the fungal metabolite eugenitin

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Abstract

Eugenitin, a chromone derivative and a metabolite of the endophyte Mycoleptodiscus indicus, at 5 mM activated a recombinant GH11 endo-xylanase by 40 %. The in silico prediction of ligand-binding sites on the three-dimensional structure of the endo-xylanase revealed that eugenitin interacts mainly by a hydrogen bond with a serine residue and a stacking interaction of the heterocyclic aromatic ring system with a tryptophan residue. Eugenitin improved the GH11 endo-xylanase activity on different substrates, modified the optimal pH and temperature activities and slightly affected the kinetic parameters of the enzyme.

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Acknowledgments

We are thankful to Dr. Janete M. Araújo from the Federal University of Pernambuco and to Dr. João Atílio Jorge from the University of São Paulo. This work was supported by Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil

    Willian J. Andrioli, Vinícius B. da Silva, Carlos H. T. P. Silva & Jairo K. Bastos

  2. Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil

    André R. L. Damásio, Alexandre Maller & Maria L. T. M. Polizeli

  3. CTBE—Laboratório Nacional de Ciência e Tecnologia do Bioetanol, CNPEM—Centro Nacional de Pesquisa em Energia e Materiais, Campinas, SP, Brazil

    André R. L. Damásio & Maria L. T. M. Polizeli

  4. Faculdade de Filosofia Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, 14040-903, Brazil

    Tony M. Silva

  5. National Center for Natural Products Research, University of Mississippi, Oxford, MS, 38677, USA

    N. P. D. Nanayakkara

Authors
  1. Willian J. Andrioli
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  2. André R. L. Damásio
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  3. Tony M. Silva
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  4. Vinícius B. da Silva
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  5. Alexandre Maller
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  6. N. P. D. Nanayakkara
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  7. Carlos H. T. P. Silva
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  8. Maria L. T. M. Polizeli
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  9. Jairo K. Bastos
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Corresponding author

Correspondence to Jairo K. Bastos.

Additional information

Willian J. Andrioli and André R. L. Damásio both contributed equally to this work.

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Andrioli, W.J., Damásio, A.R.L., Silva, T.M. et al. Endo-xylanase GH11 activation by the fungal metabolite eugenitin. Biotechnol Lett 34, 1487–1492 (2012). https://doi.org/10.1007/s10529-012-0918-3

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  • DOI: https://doi.org/10.1007/s10529-012-0918-3

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