Abstract
Recombinant human cyclooxygenase 1 (COX-1) was expressed from stably-transfected Drosophila melanogaster S2 (S2) cells. DMSO improved the expression of recombinant COX-1 by 180 %. DMSO increased the expression of nitric oxide synthase (NOS) at both the RNA and protein levels; NOS expression was closely correlated with the synthesis of recombinant COX-1 mRNA in stably-transfected S2 cells. DMSO also induced the gene encoding Kr-h1 which binds to the CACCC element of the metallothionein promoter to enhance the expression of recombinant COX-1. Therefore, DMSO improves the expression of recombinant COX-1 via NOS and/or the transcription factor Kr-h1.
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This work was supported by a grant from the Next-Generation BioGreen 21 Program (PJ008325), Rural Development Administration, Republic of Korea, and a grant from the Kyung Hee University in 2010 (KHU-20110257).
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K. H. Chang, J.-H. Park and H. Y. Chung equally contributed to this study.
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Chang, K.H., Park, JH., Chung, H.Y. et al. Enhanced expression of recombinant human cyclooxygenase 1 from stably-transfected Drosophila melanogaster S2 cells by dimethyl sulfoxide is mediated by up-regulation of nitric oxide synthase and transcription factor Kr-h1. Biotechnol Lett 34, 1243–1250 (2012). https://doi.org/10.1007/s10529-012-0911-x
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DOI: https://doi.org/10.1007/s10529-012-0911-x