Abstract
The three-dimensional (3D) plotting system is a rapidly-developing scaffold fabrication method for bone tissue engineering. It yields a highly porous and inter-connective structure without the use of cytotoxic solvents. However, the therapeutic effects of a scaffold fabricated using the 3D plotting system in a large segmental defect model have not yet been demonstrated. We have tested two hypotheses: whether the bone healing efficacy of scaffold fabricated using the 3D plotting system would be enhanced by bone marrow-derived mesenchymal stem cell (BMSC) transplantation; and whether the combination of bone morphogenetic protein-2 (BMP-2) administration and BMSC transplantation onto the scaffold would act synergistically to enhance bone regeneration in a large segmental defect model. The use of the combined therapy did increase bone regeneration further as compared to that with monotherapy in large segmental bone defects.
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This work was supported by the grant (KRF-2008-313-E00356) from the National Research Foundation of Korea.
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Sun-Woong Kang and Ji-Hoon Bae contributed equally to this work.
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Kang, SW., Bae, JH., Park, SA. et al. Combination therapy with BMP-2 and BMSCs enhances bone healing efficacy of PCL scaffold fabricated using the 3D plotting system in a large segmental defect model. Biotechnol Lett 34, 1375–1384 (2012). https://doi.org/10.1007/s10529-012-0900-0
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DOI: https://doi.org/10.1007/s10529-012-0900-0