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Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin

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Abstract

Heparin covalently attached to a water-insoluble resin suspended in HIV-infected aqueous buffer or whole blood captures the virus; subsequent physical separation of the immobilized heparin reduced the viral titers by over 80 and 50%, respectively. The detoxification concept has been validated by both circulating an HIV-1 solution through a column packed with the heparin–sepharose beads and successively mixing an HIV-1 solution with fresh beads.

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References

  • Anon (2005) Editorial: blood supply and demand. Lancet 365:2151

    Google Scholar 

  • Bryant BJ, Klein HG (2007) Pathogen inactivation: the definitive safeguard for the blood supply. Arch Pathol Lab Med 131:719–733

    PubMed  CAS  Google Scholar 

  • Bugatti A, Urbinati C, Ravelli C, De Clercq E, Liekens S, Rusnati M (2007) Heparin-mimicking sulfonic acid polymers as multitarget inhibitors of human immunodeficiency virus type 1 tat and gp120 proteins. Antimicrob Agent Chemother 51:2337–2345

    Article  CAS  Google Scholar 

  • Cecilia D, Kewal-Ramani VN, O’Leary J, Volsky B, Nyambi P, Burda S, Xu S, Littman DR, Zolla-Pazner S (1998) Neutralization profiles of primary human immunodeficiency virus type 1 isolates in the context of coreceptor usage. J Virol 72:6988–6996

    PubMed  CAS  Google Scholar 

  • De Clercq E (2007) The design of drugs for HIV and HCV. Nature Rev Drug Discov 6:1001–1018

    Article  Google Scholar 

  • Gebo KA, Fleishman JA, Conviser R, Hellinger J, Hellinger FJ, Josephs JS, Keiser P, Gaist P, Moore RD (2010) Contemporary costs of HIV healthcare in the HAART era. AIDS 24:2705–2715

    Article  PubMed  Google Scholar 

  • Hinrichs WLJ, ten Hoopen HWM, Engbers GHM, Feijen J (1997) In vitro evaluation of heparinized cuprophan hemodialysis membranes. J Biomed Mater Res 35:443–450

    Article  PubMed  CAS  Google Scholar 

  • Knipe DM, Howley PM (2001) Fields Virology, 4th edn. Lippincott Williams & Wilkins, Philadelphia

    Google Scholar 

  • Nakamura H, Miyazaki N, Hosoya N, Koga M, Odawara T, Kikuchi T, Koibuchi T, Kawana-Tachikawa A, Fujii T, Miura T, Iwamoto A (2011) Long-term successful control of super-multidrug-resistant human immunodeficiency virus type 1 infection by a novel combination therapy of raltegravir, etravirine, and boosted-darunavir. J Infect Chemother 17:105–110

    Article  PubMed  CAS  Google Scholar 

  • Nygren H (1995) Logarithmic growth in surface adsorption. Adv Colloid Interface Sci 62:137–159

    Article  PubMed  CAS  Google Scholar 

  • O’Keeffe RS, Johnston MD, Slater NKH (1999) The affinity adsorptive recovery of an infectious herpes simplex virus vaccine. Biotechnol Bioeng 62:537–545

    Article  PubMed  Google Scholar 

  • Qian K, Morris-Natschke SL, Lee K-H (2009) HIV entry inhibitors and their potential in HIV therapy. Med Res Rev 29:369–393

    Article  PubMed  CAS  Google Scholar 

  • Segura MDM, Kamen A, Trudel P, Garnier A (2005) A novel purification strategy for retrovirus gene therapy vectors using heparin affinity chromatography. Biotechnol Bioeng 90:391–404

    Article  PubMed  Google Scholar 

  • Smith PK, Mallia AK, Hermanson GT (1980) Colorimetric method for the assay of heparin content in immobilized heparin preparations. Anal Biochem 109:466–473

    Article  PubMed  CAS  Google Scholar 

  • Tullis RH, Duffin RP, Zech M, Ambrus JL (2002) Affinity hemodialysis for antiviral therapy. I. Removal of HIV-1 from cell culture supernatants, plasma, and blood. Ther Apher 6:213–220

    Article  PubMed  Google Scholar 

  • WHO and CDC data found on their respective websites (updated in July 2010): http://www.who.int/features/factfiles/hiv/en/index.html and http://www.cdc.gov/hiv/resources/factsheets/us.htm

  • Zahn A, Allain JP (2005) Hepatitis C virus and hepatitis B virus bind to heparin: purification of largely IgG-free virions from infected plasma by heparin chromatography. J Gen Virol 86:677–685

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This work was supported in part by the US army through the Institute for Soldier Nanotechnologies at the Massachusetts Institute of Technology under contract DAAD-19-02-D0002 with the Army Research Office and by National Institutes of Health grant U01-AI074443. We thanked Chia Min Lee and Alyssa Larson for insightful discussions.

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Authors and Affiliations

  1. Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Roger A. Nassar & Alexander M. Klibanov

  2. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Alexander M. Klibanov

  3. Department of Biology and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA

    Edward P. Browne & Jianzhu Chen

Authors
  1. Roger A. Nassar
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  2. Edward P. Browne
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  3. Jianzhu Chen
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  4. Alexander M. Klibanov
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Correspondence to Roger A. Nassar or Alexander M. Klibanov.

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Nassar, R.A., Browne, E.P., Chen, J. et al. Removing human immunodeficiency virus (HIV) from human blood using immobilized heparin. Biotechnol Lett 34, 853–856 (2012). https://doi.org/10.1007/s10529-011-0840-0

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  • DOI: https://doi.org/10.1007/s10529-011-0840-0

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