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Estimation of pullulan by hydrolysis with pullulanase

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Abstract

A novel method for the estimation of pullulan was developed in which pullulan was hydrolysed by pullulanase. The hydrolysed product was mainly maltotriose and was determined colorimetrically using 3,5-dimethylsalicylic acid. This gave good linearity with respect to the concentration of pullulan in the fermentation broth. The content of pullulan determined in this way was less than that determined by a coupled enzyme assay and was comparable to that determined by an HPLC method. The new method was specific for estimation of pullulan, demonstrated high accuracy, and could assay pullulan from up to 3.2 mg/ml.

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References

  • Badr-Eldin SM, El-Tayeb OM, El-Masry EG, Mohamad OA, El-Rahman OAA (1994) Polysaccharide production by Aureobasidium pullulans: factors affecting polysaccharide formation. World J Microb Biot 10:423–426

    Article  CAS  Google Scholar 

  • Deshpande MS, Rale VB, Lynch JM (1992) Aureobasidium pullulans in applied microbiology: a status report. Enzyme Microb Technol 14:514–527

    Article  CAS  Google Scholar 

  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350–356

    Article  CAS  Google Scholar 

  • Haaland PD (1989) Separating signals from the noise. In: Experimental design in biotechnology. Marcel Dekker, New York, pp 61–83

  • Israilides C, Bocking M, Smith A, Scanlon B (1994) A novel rapid coupled enzyme assay for the estimation of pullulan. Biotechnol Appl Biochem 19:285–291

    CAS  Google Scholar 

  • LeDuy A, Boa JM (1982) Pullulan production from peat hydrolysate. Can J Microbiol 29:143–146

    Article  Google Scholar 

  • Roukas T, Biliaderis CG (1995) Evaluation of carob pod as a substrate for pullulan production by Aureobasidium pullulans. Appl Biochem Biotech 55:27–44

    Article  CAS  Google Scholar 

  • Vijayendra SVN, Bansal D, Prasad MS, Nand K (2001) Jaggery: a novel substrate for pullulan production by Aureobasidium pullulans CFR-77. Process Biochem 37:359–364

    Article  CAS  Google Scholar 

  • Wu SJ, Jin ZY, Tong QY (2009) Sweet potato: a novel substrate for pullulan production by Aureobasidium pullulans. Carbohyd Polym 76:645–649

    Article  CAS  Google Scholar 

  • Yuen S (1974) Pullulan and its applications. Proc Biochem 9:7–9

    CAS  Google Scholar 

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Authors and Affiliations

  1. School of Food Engineering, HuaiHai Institute of Technology, Lianyungang, China

    Sheng-Jun Wu & Chao Zhou

  2. Food Safety and Inspection Service, USDA, 230 Washington Avenue Extension, Albany, NY, 12203, USA

    Jin-Moon Kim

  3. The State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi, China

    Zheng-Yu Jin & Qun-Yi Tong

Authors
  1. Sheng-Jun Wu
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  2. Jin-Moon Kim
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  3. Chao Zhou
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  4. Zheng-Yu Jin
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  5. Qun-Yi Tong
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Correspondence to Zheng-Yu Jin.

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Wu, SJ., Kim, JM., Zhou, C. et al. Estimation of pullulan by hydrolysis with pullulanase. Biotechnol Lett 32, 1143–1145 (2010). https://doi.org/10.1007/s10529-010-0272-2

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  • DOI: https://doi.org/10.1007/s10529-010-0272-2

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