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Growth factor production from fibrin-encapsulated human keratinocytes

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Abstract

Fibrin has been used extensively in cell encapsulation because it has important biological properties. Keratinocyte encapsulation in fibrin is a widely used technique in skin tissue engineering. The production of growth factors (EGF, TGF-β1 and PDGF-BB) was evaluated when keratinocytes are encapsulated in fibrin. Secretions of TGF-β1 and PDGF-BB increased more than five times compared to monolayer cultures. Encapsulated cells secreted about 80% active form of TGF-β1 (monolayer cells only secreted inactive form). An enhanced secretion of TGF-β1 and PDGF-BB was found in encapsulated cells, showing that fibrin capsules are favourable for the production of these growth factors.

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Acknowledgment

The authors wish to thank CONICYT by FONDEF Grant (D07I1075) and doctoral fellowships for R.A. Somoza.

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Authors and Affiliations

  1. Centro de Biotecnología, Universidad Técnica Federico Santa María, Avenida España 1680, Valparaíso, Chile

    Cristian A. Acevedo, Rodrigo A. Somoza & Manuel E. Young

  2. Departamento de Bioquímica, Facultad de Farmacia, Universidad de Valparaíso, Avenida Gran Bretaña 1093, Valparaíso, Chile

    Caroline Weinstein-Oppenheimer

  3. Departamento de Biología y Ciencias Ambientales, Facultad de Ciencias, Universidad de Valparaíso, Avenida Gran Bretaña 1111, Valparaíso, Chile

    Donald I. Brown

Authors
  1. Cristian A. Acevedo
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  2. Rodrigo A. Somoza
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  3. Caroline Weinstein-Oppenheimer
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  4. Donald I. Brown
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  5. Manuel E. Young
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Correspondence to Cristian A. Acevedo.

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Acevedo, C.A., Somoza, R.A., Weinstein-Oppenheimer, C. et al. Growth factor production from fibrin-encapsulated human keratinocytes. Biotechnol Lett 32, 1011–1017 (2010). https://doi.org/10.1007/s10529-010-0245-5

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  • DOI: https://doi.org/10.1007/s10529-010-0245-5

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