Abstract
The change in transfection efficiency of electroporation by the combined treatment with mild preheating (40°C for 30 min) was investigated. HCT 116, HeLa S3 and SGC 7901 cells were treated with electroporation in medium containing pBKCMV-Luc plasmid with or without preheating. After 24 h, luciferase activity was increased by 36, 28 and 77%; luciferase mRNA transcription was increased by 45, 50 and 68%; and fluorescein isothiocyanate-dextran accumulation was increased by 9, 35 and 15% in preheated groups, respectively. These results demonstrate that the transfection efficiency was enhanced by mild preheating. The mechanism partially involves increased macromolecular particle accumulation.
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Wei, ZL., Ogawa, R., Takasaki, I. et al. Mild hyperthermia prior to electroporation increases transfection efficiency in HCT 116, HeLa S3 and SGC 7901 cells. Biotechnol Lett 32, 367–371 (2010). https://doi.org/10.1007/s10529-009-0164-5
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DOI: https://doi.org/10.1007/s10529-009-0164-5