Abstract
Expression kinetics of six cellulase and four expansin-related genes were studied in the hypercellulolytic Trichoderma reesei CL847 mutant in response to Solka Floc cellulose and soluble inducers. Real-time PCR showed a parallel increase of transcript levels for the cellulase genes cbh1/cel7a, egl1/cel7b, egl4/cel61a, the β-glucosidase genes bgl1/cel3a, bgl2/cel1a, and the swo1 gene, encoding the cell-wall loosening protein swollenin. To evaluate a putative implication of three newly identified expansin/family 45 endoglucanase-like (EEL) proteins in lignocellulose degradation, their expression was also analysed. Only eel2 was found to be transcribed under the present conditions, and showed constitutive expression similar to the endoglucanase encoding cel5b gene.
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References
Aro N, Saloheimo A, Ilmen M et al (2001) ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei. J Biol Chem 276:24309–24314
Aro N, Pakula T, Penttila M (2005) Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiol Rev 29:719–739
Brotman Y, Briff E, Viterbo A et al (2008) Role of swollenin, an expansin-like protein from Trichoderma, in plant root colonization. Plant Physiol 147:779–789
Cantarel BL, Coutinho PM, Rancurel C et al (2009) The carbohydrate-active enzymes database (CAZy): an expert resource for glycogenomics. Nucleic Acids Res 37:D233–D238
Carle-Urioste JC, Escobar-Vera J, El-Gogary S et al (1997) Cellulase induction in Trichoderma reesei by cellulose requires its own basal expression. J Biol Chem 272:10169–10174
Durand H, Clanet M, Tiraby G (1988) Genetic improvement of Trichoderma reesei for large scale cellulase production. Enzyme Microb Technol 10:341–346
Foreman PK, Brown D, Dankmeyer L et al (2003) Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei. J Biol Chem 278:31988–31997
Fowler T, Brown RD Jr (1992) The bgl1 gene encoding extracellular beta-glucosidase from Trichoderma reesei is required for rapid induction of the cellulase complex. Mol Microbiol 6:3225–3235
Ilmen M, Saloheimo A, Onnela ML et al (1997) Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol 63:1298–1306
Mach RL, Seiboth B, Myasnikov A et al (1995) The bgl1 gene of Trichoderma reesei QM 9414 encodes an extracellular, cellulose-inducible beta-glucosidase involved in cellulase induction by sophorose. Mol Microbiol 16:687–697
Margolles-Clark E, Ilmen M, Penttila M (1997) Expression patterns of ten hemicellulase genes of the filamentous fungus Trichoderma reesei on various carbon sources. J Biotechnol 57:167–179
Martinez D, Berka RM, Henrissat B et al (2008) Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina). Nat Biotechnol 26:553–560
Mukherjee PK, Hadar R, Pardovitz-Kedmi E et al (2006) MRSP1, encoding a novel Trichoderma secreted protein, is negatively regulated by MAPK. Biochem Biophys Res Commun 350:716–722
Nogawa M, Goto M, Okada H et al (2001) l-Sorbose induces cellulase gene transcription in the cellulolytic fungus Trichoderma reesei. Curr Genet 38:329–334
Rauscher R, Wurleitner E, Wacenovsky C et al (2006) Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina. Eukaryot Cell 5:447–456
Saloheimo M, Kuja-Panula J, Ylosmaki E et al (2002a) Enzymatic properties and intracellular localization of the novel Trichoderma reesei beta-glucosidase BGLII (cel1A). Appl Environ Microbiol 68:4546–4553
Saloheimo M, Paloheimo M, Hakola S et al (2002b) Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials. Eur J Biochem 269:4202–4211
Shida Y, Furukawa T, Ogasawara W et al (2008) Functional analysis of the egl3 upstream region in filamentous fungus Trichoderma reesei. Appl Microbiol Biotechnol 78:515–524
Stricker AR, Grosstessner-Hain K, Wurleitner E et al (2006) Xyr1 (xylanase regulator 1) regulates both the hydrolytic enzyme system and d-xylose metabolism in Hypocrea jecorina. Eukaryot Cell 5:2128–2137
Yao Q, Sun TT, Liu WF et al (2008) Gene cloning and heterologous expression of a novel endoglucanase, swollenin, from Trichoderma pseudokoningii S38. Biosci Biotechnol Biochem 72:2799–2805
Acknowledgements
J. Verbeke was supported by a CIFRE scholarship from the “Association Nationale de Recherche Technique”. The authors thank Antoine Margeot for support in promoter analysis, fruitful discussions and critical remarks, as well as Frédéric Monot for revision of the manuscript.
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Verbeke, J., Coutinho, P., Mathis, H. et al. Transcriptional profiling of cellulase and expansin-related genes in a hypercellulolytic Trichoderma reesei . Biotechnol Lett 31, 1399–1405 (2009). https://doi.org/10.1007/s10529-009-0030-5
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DOI: https://doi.org/10.1007/s10529-009-0030-5