Abstract
Leymus chinensis is an important grassland perennial grass. However, its drought tolerance requires to be improved. LEA (late embryogenesis abundant) genes are believed to confer resistance to drought and water deficiency. Using Agrobacterium-mediated transformation, a wheat LEA gene, TaLEA 3 , was integrated into L. chinensis. The transgenic lines showed enhanced growth ability under drought stress during which transgenic lines had increased the relative water content, leaf water potential, relative average growth rate, but decreased the malondialdehyde content compared with the non-transgenic plant. Thus, transgenic breeding is an efficient approach to enhance drought tolerance in L. chinensis.
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References
Anonymous (1976) In: Institute of Botany, the Chinese Academy of Sciences (eds) Iconographia Cormophytorum Sinicorum. Tomus, Science Publ, Beijing7
Babu RC, Zhang J, Blum A et al (2004) HVA1, a LEA gene from barley confers dehydration tolerance in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Sci 166:855–862
Bai Y, Han X, Wu J et al (2004) Ecosystem stability and compensatory effects in the Inner Mongolia grassland. Nature 431:181–184
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Biol Sci 15:413–428
Blum A, Ebercon A (1981) Cell membrane stability as a measure of drought and heat tolerance in wheat. Crop Sci 21:43–47
Cakmak I, Horst WJ (1991) Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tips of soybean (Glycine max). Plant Physiol 83:463–468
Chen M, Li ZH, Pu SJ (1988) The observation and research on reproductive characteristics of Aneurolepidium chinensis. Res Grassl Ecosyst 2:193–208
Cuming AC (1999) LEA proteins. In: Shewry PR, Casey R (eds) Seed proteins. Kluwer Academic Publishers, The Netherlands, pp 753–780
Figueras M, Pujal J, Saleh A et al (2004) Maize Rab17 over-expression in Arabidopsis plants promotes osmotic stress tolerance. Ann Appl Biol 144:251–257
Hodges DM, DeLong JM, Forney CF et al (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611
Jiang Y, Huang B (2001) Drought and heat stress injury to cool season turfgrasses in relation to antioxidant metabolism and lipidperoxidaion. Crop Sci 41:436–442
Kruger C, Berkowitz O, Stephan UW et al (2002) A metal-binding member of the late embryogenesis abundant protein family transports iron in the phloem of Ricinus communis. Biol Chem 277:25062–25069
Leoplod AC, Willing RP (1983) Evidence of toxicity effects of salt on membranes. In: Staples RC, Toenniessen GH (eds) Plant improvement for irrigated crop production under increasing saline conditions. Wiley, New York, pp 678–685
Liang CY, Xi JY, Shu JL et al (2004) Construction of a BAC library of physcomitrella patens and isolation of a LEA gene. Plant Sci 167:491–498
Liu GS, Liu JS, Qi DM et al (2004) Factors affecting plant regeneration from tissue culture of Chinese leymus (Leymus chinensis). Plant Cell Tissue Organ Cult 76:175–178
Ma HL, Yun JF, Wan T et al (1992) The biological characteristics and performance of “Nongmu No. 1” Grassland of China 2:1–5
O’Toole JC (1982) Adaptation of rice to drought-prone environments. In: Drought resistance in crops with emphasis on rice. International Rice Research Institute, Philippines, pp 195–213
Shah NH, Paulsen GM (2003) Interaction of drought and high temperature on photosynthesis and grain-filling of wheat. Plant Soil 257:219–226
Shu QY, Liu GS, Xu SX et al (2005) Genetic transformation of Leymus chinensis with the PAT gene through microprojectile bombardment to improve resistance to the herbicide Basta. Plant Cell Rep 24:36–44
Wang W, Vinocur B, Altman A (2003) Plant response to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta 218:1–14
Xu D, Duan X, Wang B et al (1996) Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water-deficit and salt-stress in transgenic rice. Plant Physiol 110:249–257
Zhang L, Ohta A, Takagi M, Imai R (2000) Expression of plant group 2 and group 3 lea genes in Saccharomyces cerevisiae revealed functional divergence among LEA proteins. Biochem 127:611–616
Zhou G, Wang H, Wang S (2002) Responses of grassland ecosystems to precipitation and land use along the Northeast China. Transect Veg Sci 13:361–368
Acknowledgments
We are grateful to Dr Jianing Yu for the kindly supplying of TaLEA 3 gene and Dr. Guodong Wang and Dr. Haichun Jing for their critical reading of the manuscript. This research is funded by the National Basic Research Program of China (973, 2007CB108905).
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Wang, L., Li, X., Chen, S. et al. Enhanced drought tolerance in transgenic Leymus chinensis plants with constitutively expressed wheat TaLEA 3 . Biotechnol Lett 31, 313–319 (2009). https://doi.org/10.1007/s10529-008-9864-5
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DOI: https://doi.org/10.1007/s10529-008-9864-5