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Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei

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Abstract

Passiflora garckei cell cultures were used as a model to describe a reproducible sample preparation method. Solid phase extraction (SPE) was employed to isolate the plant metabolites for nuclear magnetic resonance (NMR) analysis and to subsequently detect the differences between yeast extract elicited and control cells. Compared with previous results obtained by using a Sephadex LH-20 column, SPE coupled with NMR spectroscopy improves the analysis of aromatic compounds e.g.: trans-feruloyl derivatives and trans-coumaroyl derivatives. Moreover, it decreases the concentration of sugars that usually overlap with many plant metabolite signals.

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References

  • Beecher CWW (2002) Metabolomics: a new ‘Omics’ technology. Am Genomic/Proteomic Technol 2:40–43

    CAS  Google Scholar 

  • Bitsch F, Ma W, MacDonald F, Nieder M, Shackleton CHL (1993) Analysis of taxol and related diterpenoids from cell cultures by liquid chromatography-electrospray mass spectrometry. J Chromatogr A 615:273–280

    Article  CAS  Google Scholar 

  • Dhawan K, Dhawan S, Sharma A (2004) Passiflora: a review update. J Ethnopharmacol 94:1–23

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Guzzo F, Ceolo S, Andreetta F, Levi M (2004) In vitro culture from mature seeds of Passiflora species. Sci Agric 61:108–113

    Article  CAS  Google Scholar 

  • Ketchum REB, Gibson DM (1995) A novel method of isolating taxanes from cell suspension cultures of yew (Taxus spp.). J Liq Chromatogr Relat Technol 8:1093–1111

    Google Scholar 

  • Kim HK, Choi YH, Erkelens C, Lefeberg AWM, Verpoorte R (2005) Metabolic fingerprinting of Ephedra species using 1H-NMR spectroscopy and principal component analysis. Chem Pharm Bull 53:105–109

    Article  PubMed  CAS  Google Scholar 

  • Theodoridis G, Verpoorte R (1996) Taxol analysis by high performance liquid chromatography: a review. Phytochem Anal 7:169–184

    Article  CAS  Google Scholar 

  • Vasconsuelo A, Boland R (2007) Molecular aspects of the early stages of elicitaion of secondary metabolites in plants. Plant Sci 172:861–875

    Article  CAS  Google Scholar 

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Acknowledgment

This research was partially supported by the Fondazione Cariverona, Verona, Italy.

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Authors and Affiliations

  1. Scientific and Technological Department, Faculty of Mathematics, Physics and Natural Science, University of Verona, Verona, Italy

    Marica Fraccaroli, Silvia Nicoletti, Flavia Guzzo & Marisa Levi

  2. Division of Pharmacognosy, Section of Metabolomics, Institute of Biology, Leiden University, Leiden, The Netherlands

    Federica Maltese, Young Hae Choi & Robert Verpoorte

Authors
  1. Marica Fraccaroli
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  2. Silvia Nicoletti
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  3. Federica Maltese
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  4. Young Hae Choi
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  5. Flavia Guzzo
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  6. Marisa Levi
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  7. Robert Verpoorte
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Corresponding author

Correspondence to Marica Fraccaroli.

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Fraccaroli, M., Nicoletti, S., Maltese, F. et al. Pre-analytical method for metabolic profiling of plant cell cultures of Passiflora garckei . Biotechnol Lett 30, 2031–2036 (2008). https://doi.org/10.1007/s10529-008-9785-3

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  • DOI: https://doi.org/10.1007/s10529-008-9785-3

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