Abstract
The development of microorganims that efficiently ferment lactose has a high biotechnological interest, particularly for cheese whey bioremediation processes with simultaneous bio-ethanol production. The lactose fermentation performance of a recombinant Saccharomyces cerevisiae flocculent strain was evaluated. The yeast consumed rapidly and completely lactose concentrations up to 150 g l−1 in either well- or micro-aerated batch fermentations. The maximum ethanol titre was 8% (v/v) and the highest ethanol productivity was 1.5–2 g l−1 h−1, in micro-aerated fermentations. The results presented here emphasise that this strain is an interesting alternative for the production of ethanol from lactose-based feedstocks.
References
Aguilera F, Peinado RA, Millán C, Ortega JM, Mauricio JC (2006) Relationship between ethanol tolerance, H+-ATPase activity and the lipid composition of the plasma membrane in different wine yeast strains. Int J Food Microbiol 110:34–42
Alfenore S, Cameleyre X, Benbadis L, Bideaux C, Uribelarrea J-L, Goma G, Molina-Jouve C, Guillouet SE (2004) Aeration strategy: a need for very high ethanol performance in Saccharomyces cerevisiae fed-batch process. Appl Microbiol Biotechnol 63:537–542
Compagno C, Porro D, Smeraldi C, Ranzi BM (1995) Fermentation of whey and starch by transformed Saccharomyces cerevisiae cells. Appl Microbiol Biotechnol 43:822–825
Domingues L, Teixeira JA, Lima N (1999) Construction of a flocculent Saccharomyces cerevisiae fermenting lactose. Appl Microbiol Biotechnol 51:621–626
Domingues L, Vicente AA, Lima N, Teixeira JA (2000) Applications of yeast flocculation in biotechnological processes. Biotechnol Bioprocess Eng 5:288–305
Domingues L, Lima N, Teixeira JA (2001) Alcohol production from cheese whey permeate using genetically modified flocculent yeast cells. Biotechnol Bioeng 72:507–514
Farahnak F, Seki T, Ryu DD, Ogrydziak D (1986) Construction of lactose-assimilating and high ethanol producing yeasts by protoplast fusion. Appl Environ Microbiol 51:362–367
Guimarães PMR, Virtanen H, Londesborough J (2006) Direct evidence that maltose transport activity is affected by the lipid composition of brewer’s yeast. J Inst Brew 112:203–209
Guimarães PMR, François J, Parrou JL, Teixeira JA, Domingues L (2008) Adaptive evolution of a lactose-consuming Saccharomyces cerevisiae recombinant. Appl Environ Microbiol 74:1748–1756
Kargi F, Ozmihci S (2006) Utilization of cheese whey powder (CWP) for ethanol fermentations: effects of operating parameters. Enzyme Microb Technol 38:711–718
Ozmihci S, Kargi F (2007) Kinetics of batch ethanol fermentation of cheese-whey powder (CWP) solution as function of substrate and yeast concentrations. Biores Technol 98:2978–2984
Ramakrishnan S, Hartley BS (1993) Fermentation of lactose by yeast cells secreting recombinant fungal lactase. Appl Environ Microbiol 59:4230–4235
Rigoulet M, Aguilaniu H, Averet N, Bunoust O, Camougrand N, Grandier-Vazeille X, Larsson C, Pahlman IL, Manon S, Gustafsson L (2004) Organization and regulation of the cytosolic NADH metabolism in the yeast Saccharomyces cerevisiae. Mol Cell Biochem 256–257:73–81
Rubio-Texeira M (2006) Endless versatility in the biotechnological applications of Kluyveromyces LAC genes. Biotechnol Adv 24:212–225
Rubio-Texeira M, Castrillo JI, Adam AC, Ugalde UO, Polaina J (1998) Highly efficient assimilation of lactose by a metabolically engineered strain of Saccharomyces cerevisiae. Yeast 14:827–837
Rubio-Texeira M, Arevalo-Rodriguez M, Lequerica JL, Polaina J (2000) Lactose utilization by Saccharomyces cerevisiae strains expressing Kluyveromyces lactis LAC genes. J Biotechnol 84:97–106
Siso MIG (1996) The biotechnological utilization of cheese whey: a review. Biores Technol 57:1–11
Sreekrishna K, Dickson RC (1985) Construction of strains of Saccharomyces cerevisiae that grow on lactose. Proc Natl Acad Sci USA 82:7909–7913
Verduyn C, Postma E, Scheffers WA, van Dijken JP (1992) Effect of benzoic acid on metabolic fluxes in yeasts: a continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8:501–517
Acknowledgements
We thank Jaroslav Klein for assistance in the bioreactor fermentations and Marisa Cunha for doing the HPLC analyses of some of the experiments. The financial support of Fundação para a Ciência e a Tecnologia (FCT), Portugal (project ProBioethanol PTDC/BIO/66151/2006) is acknowledged. P. M. R. Guimarães acknowledges support from FCT, Portugal (grant SFRH/BD/13463/2003).
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Guimarães, P.M.R., Teixeira, J.A. & Domingues, L. Fermentation of high concentrations of lactose to ethanol by engineered flocculent Saccharomyces cerevisiae . Biotechnol Lett 30, 1953–1958 (2008). https://doi.org/10.1007/s10529-008-9779-1
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DOI: https://doi.org/10.1007/s10529-008-9779-1