Abstract
A new full-length cDNA (Ssd12) encoding a Δ12-fatty acid desaturase (Δ12-FAD) was cloned from Sapium sebiferum using RT-PCR and RACE methods. Ssd12 contained a 1146 bp open reading frame encoding a protein of 381 amino acids. The amino acid sequence showed a much higher match with microsomal Δ12-FAD amino acid sequences than chloroplast Δ12-FAD amino acid sequences. Genomic Southern blot analysis suggested that Ssd12 had at least two copies. Ssd12 transcripts were detected in roots, leaves, stems, and seeds by real time PCR.
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We thank Prof. Yi Yang, Dr. Nianhui Zhang, Dr. Juan Li, and Dr. Emily H. King for their help in correction to the language.
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Niu, B., Ye, H., Xu, Y. et al. Cloning and characterization of a novel Δ12-fatty acid desaturase gene from the tree Sapium sebiferum . Biotechnol Lett 29, 959–964 (2007). https://doi.org/10.1007/s10529-007-9325-6
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DOI: https://doi.org/10.1007/s10529-007-9325-6